钛表面微-纳米结构改性对变形链球菌粘附的影响  

Effects of micro-nano patterned surface modification on Streptococcus mutans adhesion

作　　者：刘雯君[1] 邱憬[1] 仇颖莹 杨洁[1] 王国平[1] 汤春波[1] 

机构地区：[1]南京医科大学口腔医学研究所南京医科大学附属口腔医院种植修复科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2015年第5期727-731,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81472928);江苏省自然科学基金(BK20130898);江苏省高校大学生创新计划项目(201310312020Z);江苏高校优势学科建设工程资助项目(2014-37)

摘　　要：目的 :研究钛表面微-纳米结构改性对变形链球菌粘附的影响。方法 :根据钛表面处理方法分为4组:机械抛光组、喷砂组、阳极氧化组、喷砂-阳极氧化组。采用扫描电镜观察各组试件的表面形貌,采用接触角测量仪测算双蒸水在试件表面的接触角,检测各组试件表面的润湿性。将试件与变形链球菌共同孵育,采用菌落形成单位计数法和扫描电镜观测试件表面粘附细菌的数量和形态。结果:扫描电镜观察显示,喷砂-阳极氧化组的钛表面形成微米孔内复合纳米管阵列的微-纳米结构。四组试件表面的接触角为:机械抛光组、喷砂组>阳极氧化组、喷砂-阳极氧化组。4组试件表面的变形链球菌粘附数量为:阳极氧化组、喷砂-阳极氧化组>喷砂组>机械抛光组。结论:与钛表面纳米管阵列相比,钛表面微-纳米结构改性未增加变形链球菌的粘附数量。Objective：To evaluate the effects of micro-nano patterned titanium surface modification on Streptococcus mutans adhesion. Methods：According to different titanium surface modifications,there were four groups：Mechanical polishing group, Sandblasting group, Anodic oxidation group, and Sandblasting-anodic oxidation group. The specimen of each group were characterized by scanning electron microscopy. The contact angles of double distilled water on specimen of each group were calculated to evaluate the wettabilities. The specimens were incubated in Streptococcus mutans cell suspension,and the bacteria on specimens were numbered by colony-forming units and observed by scanning~ electron microscopy. Results：Scanning electron microscope demonstrated that the micro-nano patterned structure of nano-tube arrays within micro-pores grew on the titanium surface of Sandblasting-anodic oxidation group. The sequence of surface contact angles among four groups was as follows：Mechanical polishing group ,Sandblasting group 〉 Anodic oxidation group, Sandblasting-anodic oxidation group. The sequence of the bacterial adhesion quantities among four groups was as follows：Anodic oxidation group,Sandblasting-anodic oxidation group 〉 Sandblasting group 〉 Mechanical polishing group. Conclusion： In comparison with the nano-tube arrays,the micro-nano patterned surface modification did not increase the adhesion of Streptococcus mutans on the titanium surface.

关 键 词：钛 微-纳米结构 变形链球菌 

分 类 号：R783.1[医药卫生—口腔医学]