肿瘤标志物CD133和CD166在结直肠癌中的表达及临床意义  

Expression and its clinical significance of cancer markers CD133 and CD166 in colorectal carcinoma

作　　者：祝骁涛[1] 黄建[2] 李曙光[1] 陆晓峰[1] 胡斌[3] 

机构地区：[1]金华市中心医院肿瘤科,浙江金华321000 [2]浙江大学附属第二医院肿瘤科,浙江杭州310000 [3]金华市中心医院病理科,浙江金华321000

出　　处：《中国卫生检验杂志》2015年第11期1692-1695,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨肿瘤干细胞标志物CD133和CD166在结直肠癌中的表达及其临床意义。方法采用免疫组织化学方法检测102例结直肠癌患者的癌组织、40例结直肠癌患者的癌旁正常黏膜组织及40例健康人群的结肠腺瘤组织中CD133和CD166的表达,分析其与结直肠癌临床病理参数间的关系。结果 102例结直肠癌患者的癌组织中,CD133和CD166的阳性率分别为71.6%和60.8%,而对照组结肠腺瘤中的阳性染色率分别为17.5%和15.0%,正常黏膜组织阳性率均为0%,两两比较差异均具有统计学意义(P<0.05)。CD133和CD166蛋白的表达与结直肠癌的淋巴结转移和术后复发转移有相关性(P<0.05)。CD133和CD166双阳性组的5年生存率为42.5%,双阴性组为75.0%,差异具有统计学意义(P<0.05)。结论 CD133和CD166在结直肠癌癌组织中的表达高于腺瘤组织和癌旁组织,两者阳性组与预后呈负相关,提示其可能与结直肠癌的恶性演进有关,可作为结直肠癌侵袭、淋巴结转移和预后的重要标志。Objective To investigate the expression and the clinical significance of cancer markers CD133 and CD166 in color- ectal carcinoma. Methods The expression of CD133 and CD166 was detected by immunohistochemistry in 102 cases of color- ectal carcinoma, and in 40 cases of adjacent normal colorectal mucosa, and in 40 cases of colorectal adenoma tissues. Its asso- ciation with clinieopathologieal parameters was analyzed. Results The positive rate of CD133, and CD166 expression were 71.6%, and 60.8% respectively in 102 cases of colorectal carcinoma. In control group, its positive rate in 40 colorectal adeno- ma tissues and adjacent normal colorectal mueosa were 17.5% , 15.0% , and 0% , respectively. The differences between the two groups were statistically significant （ P 〈 0.05 ）. The expression of CD133, and CD166 were correlated with lymph node me- tastasis, and postoperative recurrence （ P 〈 0.05 ）. The five - year survival rate of the both positive rate of CD133 and CD166 expression was 42.5%, the both negative rate of there was 75.0% , and the difference had statistical significance （ P 〈 0.05 ）. Conclusion Expression of CD133 and CD166 in colorectal carcinoma was significantly higher than that in adenoma and carci- noma tissues, both positive group and prognosis were negatively correlated. It is suggested that it may be associated with the ma- lignant progression of colorectal cancer, which can be an important symbol of colorectal cancer invasion, metastasis and prognosis of lymph node.

关 键 词：肿瘤干细胞 结直肠癌 CD133 CD166 

分 类 号：R735.3[医药卫生—肿瘤]