气相色谱-质谱、气相色谱-火焰光度、气相色谱-氮磷3种检测器测定毒鼠强的方法探讨  

Comparison of the determination of tetramine by GC-MS,GC-FPD(S),GC-NPD three detectors

作　　者：郑三燕[1] 吴俊[1] 林少美[1] 

机构地区：[1]温州市疾病预防控制中心,浙江温州325000

出　　处：《中国卫生检验杂志》2015年第11期1719-1721,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的比较气相色谱-质谱(GC-MS)、气相色谱-火焰光度检测器[GC-FPD(S)]、气相色谱-氮磷检测器(GC-NPD)3种不同检测器测定毒鼠强的优、缺点。方法固体样品经过乙酸乙酯萃取,经氮吹浓缩后,提取物质分别用气相色谱-质谱、气相色谱-火焰光度检测器、气相色谱-氮磷检测器检测,比较3种检测器的灵敏度和定性、定量能力。结果在测定高浓度毒鼠强时,MS的灵敏度最高,FPD(S)次之,NPD的灵敏度最低。在测定低浓度时,MSSCAN、FPD(S)、NPD的灵敏度均下降,而MS-SIM模式干扰小,灵敏度得到很大改善。结论经过比较,质谱在毒鼠强定性方面有其独特的优点,采用全扫描模式,可以对毒鼠强进行结构定性,亦可用SIM模式进行定量。而FPD(S)、NPD根据保留时间来定性毒鼠强,容易出现假阳性,故采用双柱来定性。Objective To compare the differences of gas chromatography - mass spectrometry （ GC - MS）, gas chromatography with flame photometric detector[ GC -FPD（S） ], gas chromatography with nitrogen phosphorus detector（ GC -NPD） three dif- ferent detectors detecting the tetramine. Methods Solid samples were extracted by ethyl acetate, concentrated by nitrogen blo- wing, the extractions were detected by GC -MS, GC -FPD（ S）, GC -NPD, and their sensitivity, qualitative and quantitative ability were compared. Results At the high concentration of the tetramine, the highest sensitivity was MS, the sensitivity of FPD（S） was second, NPD was the lowest. At the low concentration, the sensitivity of MS -SCAN, FPD （S） and NPD were dccreased, whilc the sensitivity of MS - SIM mode with little disturbs was greatly improved. Conclusion After the comparison, mass spectrometry in tetramine qualitative, have their own unique advantages, using full scan mode, which can be qualitatively on tetramine, SIM model is also available for quantitative. While FPD （S） and NPD, according to retention time, quantify tetramine, which is prone to have false positive, so it＇ d better use double column to quantify.

关 键 词：毒鼠强 气相色谱-质谱 气相色谱-火焰光度检测器 气相色谱-氮磷检测器 

分 类 号：O657.7[理学—分析化学]