牛白藤的化学成分及其免疫抑制活性研究  

Chemical constituents from stems of Hedyotis hedyotidea and their immunosuppressive activity

作　　者：张甜甜[1] 高莎莎[1] 侯俊杰 周永芹[3] 周杰文[1] 王小刚[1] 秦楠[4] 陈家春[1] 段宏泉[4] 方进波[1] 

机构地区：[1]华中科技大学同济医学院药学院,天然药物化学与资源评价湖北省重点实验室,湖北武汉430030 [2]湖北省食品药品监督检验研究院,湖北武汉430064 [3]华中科技大学同济医学院基础医学院,湖北武汉430030 [4]天津医科大学药学院,基础医学研究中心,天津300070

出　　处：《中国中药杂志》2015年第12期2357-2362,共6页China Journal of Chinese Materia Medica

摘　　要：研究茜草科耳草属植物牛白藤Hedyotis hedyotidea（DC.）Merr.干燥藤茎的化学成分及免疫抑制活性,为进一步开发利用牛白藤提供依据。以硅胶、凝胶柱色谱、制备高效液相色谱进行分离纯化,并应用MS和NMR技术分析鉴定化合物结构,分离得到11个化合物吐叶醇（6S,9S-vomifoliol,1）,桦木酮酸（betulonic acid,2）,白桦脂酸（betulinic acid,3）,白桦醇（betulin,4）,表白桦脂酸（3-epi-betulinic acid,5）,乌苏酸（ursolic acid,6）,β-谷甾醇（β-sitosterol,7）,stigmast-4-en-3-one（8）,7β-hydroxysitosterol（9）,（3β,7β）-7-methoxystigmast-5-en-3-ol（10）和巴戟醚萜（morindacin,11）。化合物1,2,4,8-11均首次从该植物中分得,化合物1,2,8-11均首次从耳草属植物中分得,化合物9,10均首次从茜草科植物中分得。通过体外淋巴细胞转化实验测试化学成分的免疫抑制活性,化合物4,6,9均有较强的免疫抑制活性。Hedyotis hedyotidea has been traditionally used for the treatment of arthritis,cold,cough,gastro-enteritis,headstroke,etc. But few studies have screened the active compounds from extracts of H. hedyotidea. In this study,the structure of the chemical constituents from stems of H. hedyotidea were determined and the immunosuppressive activity of the compounds was evaluated. The compounds were separated and purified with silica gel,gel column chromatographies and preparative HPLC,and their structures were identified by spectral methods such as MS and NMR. Eleven compounds were obtained and identified as（6S,9S）-vomifoliol（1）,betulonic acid（2）,betulinic acid（3）,betulin（4）,3-epi-betulinic acid（5）,ursolic acid（6）,β-sitosterol（7）,stigmast-4-en-3-one（8）,7β-hydroxysitosterol（9）,（3β,7β）-7-methoxystigmast-5-en-3-ol（10） and morindacin（11）. This is the first report of compounds 1,2,4,8,9,10 and 11 from H. hedyotidea. Compounds 1,2 and 8-11 were firstly isolated from the genus Hedyotis,and compounds 9 and10 were isolated from the family Rubiaceae for the first time. The immunosuppressive activity of these compounds was tested using the lymphocyte transsormationtest. Compounds 4,6 and 9 showed significant immunosuppressive activity.

关 键 词：牛白藤 (6S 9S)-vomifoliol 环烯醚萜 三萜 甾体 免疫抑制活性 

分 类 号：R284.1[医药卫生—中药学]