多西他赛对小鼠肺转移黑色素瘤抑制作用的研究  

Study on the Inhibitory Effect of Docetaxel on Mouse Melanoma with Lung Metastasis

作　　者：胡华 

机构地区：[1]武汉市161医院药械科,湖北武汉430012

出　　处：《标记免疫分析与临床》2015年第6期555-558,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨多西他赛(多西紫杉醇)对小鼠B16-F10黑色素瘤肺转移的抑制作用。方法 MTT法检测多西他赛对小鼠B16-F10细胞生长能力的影响;细胞粘附性实验研究多西他赛对小鼠B16-F10细胞粘附能力的影响;体内实验采用C58BL/6小鼠尾静脉注射B16细胞建立实验性肺转移模型,观察腹腔注射多西他赛对小鼠肺组织肿瘤肺转移结节的影响。结果 20μg/m L多西他赛对小鼠B16-F10细胞的体外生长有一定的抑制作用,并且能够显著降低B16-F10细胞同基质的粘附能力(P<0.05)。l5μg/m L、10μg/m L、20μg/m L的多西他赛24h对小鼠体外B16-F10细胞的抑制率分别是2.34%、16.27%和41.63%。荷黑色素瘤B16-F10肺转移癌小鼠经腹腔注射5μg/m L、10μg/m L、20μg/m L三个剂量的多西他赛对肿瘤的抑制率分别为42.70%、15.47%和5.45%,高剂量组(20μg/m L)显示对肺转移黑色素瘤有明显的抑制作用(P<0.05)。结论多西他赛能够抑制小鼠肺转移黑色素瘤,其机制可能与其对B16-F10细胞的粘附能力和生长能力的抑制作用有关。Objective To investigate the inhibitory effect of docetaxel （Taxotere） on murine B16- F10 melanoma with lung metastasis. Methods The effect of docetaxel on cell growth and cell adhesion ability were detected by MTT method and cell adhesion experiment on mouse B16-F10 cells respectively. The mouse melanoma with pulmonary metastasis models were established with C58BL/6 mice by tail vein injection of B16-F10 cells and in vivo experiment were carried out to observe the effects of abdominal cavity injection of docetaxel on tumor metastasis of lung nodules in the lung tissue of mice. Results The in vitro cell studies showed that 20μg/mL of docetaxel had certain inhibitory effect on mouse B16-F10 cells growth and significantly reduced the adhesion ability of B16-F10 cells with matrix（P 〈0.05）. The in vitro inhibition rate on B16-F10 cells growth with 5μg/mL, 10μg/mL and 20μg/mL of docetaxel at 24 h were 2.34%, 16.27% and 41.63% respectively. The tumor inhibition rate with abdominal cavity injection of 5 μg/mL, 10μg,/mL and 20μg/mL of docetaxel in mouse melanoma with pulmonary metastasis were 42.7%, 15.47% and 5.45% respectively. Conclusion Docetaxel could suppress the mouse melanoma with lung metastasis, and the mechanism may be related to its inhibitory effect on B16-F10 cell adhesion ability and growth ability.

关 键 词：多西他赛 实验性肺转移 黑色素瘤 MTT法 细胞粘附性 

分 类 号：R734.2[医药卫生—肿瘤]