酶制剂浓缩方法研究进展  

Progress of concentrating enzyme

作　　者：白净[1] 黄会杰[1] 陈俊英[1] 常春[1] 方书起[1] 李洪亮[1] 张璐[1] 闫德冉 

机构地区：[1]郑州大学化工与能源学院,河南郑州450001 [2]车用生物燃料技术国家重点实验室,河南南阳473000

出　　处：《化工进展》2015年第6期1526-1531,共6页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金(U1404519);河南省科技攻关计划(132102310042);车用生物燃料技术国家重点实验室开放基金(KFKT2013012)项目

摘　　要：目前酶制剂生产过程中常用的浓缩方法存在工艺复杂、能耗较高、成本高昂等缺点,并成为酶制剂工业进一步发展的关键步骤。本文综述了蒸发、超滤、吸附和冷冻等常用的酶制剂浓缩方法,以及较新的离子液体和水合物浓缩酶制剂方法等,并比较了这些方法的优缺点。蒸发浓缩酶制剂技术成熟,但是能耗较高、酶失活率高。超滤和吸附浓缩酶制剂方法能耗较低,但是膜和吸附材料成本较高、再生困难。冷冻酶制剂浓缩方法酶失活少,但是浓缩率低。离子液体酶制剂浓缩方法具有保持酶活性、易于放大、可连续操作等优点,但是技术仍不成熟。水合物酶制剂浓缩方法目前尚处于实验室研究阶段。因此,开发出工艺简单、性能可靠、能耗较低、成本低廉的高纯度酶制剂浓缩方法依然是未来的研究方向。Presently,the enzyme concentrating method as the critical procedure in production of enzyme has many disadvantages,including complex process and high energy consumption. The common methods,including evaporation,ultrafiltration,adsorption,freezing are compared with the latest methods,including ionic liquid,hydrate,and their advantages and disadvantages are analyzed. Evaporation technology is mature but consumes excessive energy and keeps less enzyme activity. Ultrafiltration and adsorption consume less energy but have high material costs and are difficult to regenerate. The disadvantage of freezing concentration is the limit of concentration,though it can keep more activity. Concentrating by the ionic liquid method is easy to scale up and has the advantage of continuous operation,but the technology is not mature enough. The hydrate method is still in the laboratory. So developing a method of simple process,reliable performance,low energy consumption and low cost will be a research direction in the future.

关 键 词：酶制剂 浓缩 分离 进展 

分 类 号：TQ925[轻工技术与工程—发酵工程]