金属组学方法研究生物体内汞和硒相互作用  

Investigation of mercury-selenium interaction in bio-organisms using metallomics approach

作　　者：赵甲亭 李玉锋[1] 朱娜丽[2] 高愈希[1] 柴之芳[1] 

机构地区：[1]中国科学院高能物理研究所核辐射与核能技术重点实验室纳米生物效应与安全性重点实验室,北京100049 [2]中国科学院生物物理研究所蛋白质科学研究平台蛋白质组学技术实验室,北京100101

出　　处：《科技导报》2015年第12期93-100,共8页Science & Technology Review

基　　金：国家自然科学基金项目(21407150;11205168;11375213);国家自然科学基金-大科学装置联合基金重点支持项目(U1432241)

摘　　要：随着金属组学及金属组学研究技术的不断发展,近年来,金属组学研究技术,如各种核分析技术,同位素稀释(示踪)技术、同步辐射X射线荧光分析(SR-XRF)、同步辐射X射线吸收光谱(SR-XAS)技术等,结合电泳、色谱、质谱等生化分离、分析技术,应用于研究生物体内硒和汞的生物学行为及相互作用机制,将该领域研究推向一个新的高度。硒是人体及动物维系生命活动所必需的微量元素之一,对汞等重金属的毒性具有显著的拮抗作用。全面准确地了解生物体内汞和硒的分布和存在形式,研究生物组织中汞的吸收、迁移、转化和蓄积过程以及硒对汞的这些生物学行为和效应的影响,对于环境汞污染和汞毒性危害的控制具有现实意义,同时有助于理解生物体内汞和硒的相互作用过程和机制。本文综述常用的金属组学方法在植物、动物和人体内汞和硒相互作用方面的研究进展。As metallomics becomes a focus area in recent years, the related research approaches are getting a rapid development. Especially for the nuclear analysis techniques, such as isotope dilution （tracer） technique, synchrotron radiation X ray fluorescence analysis （SR-XRF）, and synchrotron radiation X ray absorption spectroscopy （SR-XAS） techniques, combined with other biochemical separation and analysis techniques like electrophoresis, high performance liquid chromatography-inductively coupled plasma mass spectrometry （HPLC-ICP-MS）, electrospray ionization mass spectrometry （ESI-MS） and so on, they have been widely used for the function and interaction research of Hg and Se in bio-organisms. Selenium （Se） is one of the essential trace elements for human and animals, and can apparently antagonize the toxicity of heavy metals such as mercury （Hg）. To analyze the distribution and speciation of Se and Hg in organisms, to get a insight into their absorption, transformation and accumulation in different tissues, and to further explore the potential roles of Se on the bio-effects of Hg in organisms is quite crucial for the control of Hg pollution and the acquaintance with Hg-Se interaction in bio-organisms. Based on the previous reports and the results from our lab, the present work will give an informative review about the metallomics approaches as applied in the research of Hg, Se interaction in bio-organisms.

关 键 词：汞 硒 金属组学 核分析技术 

分 类 号：X17[环境科学与工程—环境科学]