培养条件对小球藻生长及油脂积累的影响研究  

The impact of different culture conditions on the chlorella's growth and fat accumulation

作　　者：周连宁[1,2] 王波[1] 吴锋[1] 赵振业[1,2] 梅立永[1] 

机构地区：[1]深圳市深港产学研环保工程技术股份有限公司,广东深圳518057 [2]深港产学研基地,广东深圳518057

出　　处：《生物学杂志》2015年第3期46-50,共5页Journal of Biology

基　　金：深圳市战略新兴产业发展专项资金项目(CXZZ20120618111150009);青年科学基金项目(513080061006239)

摘　　要：随着化石能源枯竭,微藻生物柴油日益成为研究热点,不同的培养条件对小球藻的生长及油脂积累有很大的影响。因此考察了温度、光强和水体p H值等因素对小球藻的生长及油脂积累的影响。结果表明,不同温度下小球藻的生长情况和油脂含量不同。在25℃时,小球藻细胞密度最大,油脂积累量最高,达到了26.50%±4%;光照强度对小球藻生长及油脂积累也有很大影响,5000 lux时小球藻细胞密度最大,油脂含量最高;同时p H值对小球藻生长及油脂积累也有显著作用,在p H值为7时小球藻细胞密度和油脂积累量最大,其中油脂积累量为(23.96±4.3)%。With the depletion of fossil fuels, algae biodiesel increasingly become a hot topic, and a different culture conditions have a great impact on the growth and fat accumulation of Chlorella. Therefore, this paper examined the impact of factors such as tempera-ture, light intensity, and water pH on the Chlorella’s growth and fat accumulation. The results showed that Chlorella’s growth at dif-ferent temperatures have different fat accumulation. At 25℃, Chlorella cells reached the highest density, and the highest accumula-tion of grease could reach to （26.50 ＆#177; 4）%;light intensity also had a great impact on Chlorella’s growth and grease accumulation, and Chlorella the maximum cell density, highest fat content appeared at 5000 lux;pH also have a significant role in the growth of Chlorella and fat accumulation, when pH=7, the Chlorella cells reached to the highest density and the highest accumulation of grease-reached to （23.94 ＆#177; 4.3）%.

关 键 词：培养条件 小球藻 油脂含量 

分 类 号：S968.49[农业科学—水产养殖] Q949.2[农业科学—水产科学]