树脂D202对埃博霉素发酵产量的影响  

Effect of Resin D202 on Fermentation Yield of Epothilones

作　　者：李灿[1] 赵林[1] 王晓娜[1] 任强[1] 和富明 刘新利[1] 

机构地区：[1]齐鲁工业大学山东省微生物工程重点实验室,山东济南250353

出　　处：《食品与药品》2015年第3期175-179,共5页Food and Drug

基　　金：省级博士后创新基金会(201203006);山东省科技支撑计划项目(2012GSF12107)

摘　　要：目的研究离子交换树脂D202对合成埃博霉素发酵产量的影响。方法利用硝酸银滴定法和酶活测定法研究加入树脂前后各参数的变化。结果加入阴离子交换树脂D202可降低发酵培养基中氯离子含量,进一步影响纤维堆囊菌三羧酸循环中α-酮戊二酸脱氢酶复合体(OGDHC)活性。结论树脂D202可显著促进纤维堆囊菌生长,进而提高埃博霉素的产量。Objective To study the effect of anion exchange resin D202 on the fermentation yield of epothilones. Methods The changes of the parameters before and after addition of resin D202 were determined by silver nitrate titration and enzyme activity determination. Results The addition of resin D202 could lower the content of chloride ion in the fermentation medium, and the depressed chloride ion content further impacted the activity of oxoglutarate dehydrogenase complex （OGDHC）. Conclusion Resin D202 can significantly promote the growth of Sorangium cellulosum, and increase the production of epothilones.

关 键 词：纤维堆囊菌 埃博霉素 α-酮戊二酸脱氢酶复合体 树脂D202 

分 类 号：R979.14[医药卫生—药品] TQ927[医药卫生—药学]