Netrin-1受体UNC5C广泛甲基化与大肠癌的相关性分析  

Correlation analysis of Netrin-1 receptor UNC5C wide methylation and colorectal cancer

作　　者：孙冬生[1] 贾凤杰 刘宁[1] 王刚[1] 李海洋[1] 高春艳[1] 王志钢[2] 

机构地区：[1]河北省唐山市开滦总医院,河北唐山063000 [2]内蒙古大学生命科学学院,内蒙古呼和浩特010021

出　　处：《现代中西医结合杂志》2015年第18期1964-1965,2015,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：中国煤炭工业协会2012年度科学技术研究指导性计划项目(MTKJ2012-420)

摘　　要：目的：探讨神经突起生长导向因子（ Netrin -1）受体 UNC5C 广泛甲基化与大肠癌发生、发展的相关性。方法使用结合重亚硫酸盐限制性内切酶法（ COBRA）检测54例散发性大肠癌组织及其相关正常黏膜组织以及52例大肠腺瘤组织中 UNC5C 启动子的2个区域（区域1和区域2）中 UNC5C甲基化情况，分析 UNC5C 甲基化与大肠癌发病的相关性。结果广泛 UNC5C甲基化（区域1和区域2均甲基化）及部分甲基化（区域1或区域2甲基化）在大肠癌组织、腺瘤组织及正常黏膜组织中分别占59%（32/54）和87%（47/54），8%（4/52）和38%（20/52），0%（0/54）和9%（5/54）。无论广泛还是部分 UNC5C 的甲基化率大肠癌组织均明显高于腺瘤组织及正常组织（P均﹤0.05）。UNC5C 基因在Ⅰ～Ⅱ期以及Ⅲ～Ⅳ期的甲基化水平均显著高于未甲基化水平，Ⅰ～Ⅱ期甲基化水平与Ⅲ~Ⅳ期甲基化水平相比差异无统计学意义（P均﹥0.05）。UNC5C甲基化与大肠癌发病呈显著正相关（r＝0.856，P ﹤0.05），但广泛甲基化与肿瘤的分期并无明显相关性。结论 Netrin-1受体 UNC5C甲基化及其范围与大肠癌发生具有紧密联系，值得临床重视。Objective It is to approach the correlation of Netrin-1 receptor UNC5C wide methylation and colorectal canc-er in occurrence,development of colorectal cancer. Methods Methylation state of UNC5C in two regions of the promoter of UNC5C（zones 1 and 2）in 54 patients with sporadic colorectal cancer related normal mucosa,and 52 cases of colorectal ade-noma tissue by using combined bisulfite restriction endonuclease method（COBRA）,than the correlation of UNC5C methyla-tion and incidence of colorectal cancer. Results The rate of extensive methylation of UNC5C（region 1 and region 2 methyla-tion）and partial methylation（ zone 1 or zone 2 methylated）in Colorectal cancer,adenoma and normal mucosa tissues is 59%（32/54）and 87%（47/54）,8%（4/52）and 38%（20/52）,0%（0/54）and 9%（5/54）respectively. Whether widely or part methylation rate of UNC5C in colorectal cancer was significantly higher in adenoma and normal mucosa tissues（all P﹤0. 05）. The methylation levels of UNC5C gene in Ⅰ to Ⅱ stage and Ⅲ to Ⅳ stage were significantly higher than those of no methylation,there was no significant difference in methylation level between Ⅰ to Ⅱ stage and Ⅲ to Ⅳ stage（P ﹥0. 05）. UNC5C methylation was positively correlated with the incidence of colorectal cance（r=0. 856,P﹤0. 05）,but there was no correlation between extensive methylation and tumor stage. Conclusion Netrin-1 receptor UNC5C methylation and its range is close contacted to the colorectal diseases,it is worthy of attention.

关 键 词：Netrin-1受体 UNC5C甲基化 广泛甲基化 部分甲基化 大肠癌 

分 类 号：R735.204[医药卫生—肿瘤]