维生素D3对小鼠肝脏病理学及肝纤维化指标的影响  

Effects of vitamin D3 on liver pathology and fibrotic profiles of mice experimental hepatic injury

作　　者：王家健[1] 蒋红梅[1] 胡朝晖[2] 黄献中[2] 谷俊莹[1] 

机构地区：[1]贵阳医学院医学检验学院,贵阳550004 [2]广州金域医学检验中心,广州510330

出　　处：《检验医学与临床》2015年第12期1710-1712,1715,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨维生素D3(VitD3)对四氯化碳(CCl4)诱导雌性BALB/C小鼠急性肝损伤(acute liver injury)中肝纤维化指标的影响及其病理学改变。方法将25只SPF级雌性BALB/C小鼠随机分为正常对照组、模型组及VitD3高、中、低剂量干预组。VitD3高、中、低剂量组分别每日给予VitD3 15.0μg、7.5μg、l.0μg腹腔注射,正常对照组与模型组腹腔注射等量生理盐水,预处理2周。VitD3预处理1周后,模型组与VitD3高、中、低剂量组每只小鼠隔日腹腔注射0.1%CCl4橄榄油溶液0.2mL造模,正常对照组与模型组同步隔日腹腔注射生理盐水,CCl4给药7d后,全部小鼠经麻醉眼眶采血,分离肝脏并称质量,光镜下观察肝组织HE、Masson及网状纤维染色的病理变化;常规生化方法检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST);放射免疫法检测血清透明质酸(HA)、Ⅲ型前胶原N端肽(PⅢNP)、Ⅳ型胶原(CⅣ)、层粘连蛋白(LN)。结果与正常对照组比较,模型组BALB/C小鼠肝脏指数和血清ALT、AST、HA、LN、PⅢNP、CⅣ皆显著升高,差异有统计学意义(P<0.01)。与模型组比较,VitD3高、中剂量组的肝脏指数、ALT、AST、HA、LN、PⅢNP、CⅣ均显著降低,差异有统计学意义(P<0.01)。结论 VitD3能有效减轻CCl4诱导急性肝损伤小鼠肝组织的炎性活动度和纤维化程度,提示VitD3具有一定的抗炎、抗纤维化作用。Objective To investigate the effects of vitamin D3 on liver pathology and fibrotic profiles,acute liver injury was produced in BALB/C mice by CCl4.Methods Twenty five BALB/C mice were randomly and equally divided into five groups：control group,vehicle group,and three vitamin D3groups（n=5for each group）.Doses of vitamin D3 were 15μg,7.5μg,1μg in the vitamin D3groups（daily,intraperitoneal）,which were indicated as high,medium and low does.Mice in control group and vehicle group were daily intraperitoneal injected with 0.9%Nacl.After the two weeks treatment,vehicle group and treatment groups were intraperitoneal injected with 0.1%CCl4in olive（0.2mL/2d,ip）7days later.Meanwhile,the control group was equivalently intraperitoneal injected with 0.9%Nacl daily.At the end of administration of CCl4 solution,blood and liver tissue were harvested from all the mice.Pathological changes in liver tissues were analyzed by HE staining,reticular fiber staining and Masson staining.Alanine aminotransferase（ALT）and Aspartate transaminase（AST）were detected with routine biochemical method.Hyauline（HA）,collagen typeⅣ（IVC）,laminin（LN）,and collagen typeⅢN-terminal peptide（PⅢNP）were detected with RIA.Results The levels of liver index,ALT,AST,HA,LN,PⅢ NP,CⅣ were significantly higher in the vehicle group than that in the control group（all P〈0.01）.Compared with vehicle group,the levels of serum liver index,ALT,AST,HA,LN,PⅢ NP,CⅣ were significantly debased after the treatment with vitamin D3（all P〈0.01）.Conclusion Vitamin D3 has anti-inflammation and anti-fibrosis effects in the mice experimental liver injuries induced by CCl4,by reducing level of serum fibrosis in early liver fibrosis.

关 键 词：维生素D3 肝损伤 肝纤维化 

分 类 号：R57[医药卫生—消化系统]