ER-α36在2种人胶质瘤细胞中的表达比较及与Tamoxifen抗药性关系初探  

Diverse expression of ER-α36 in two kinds of human glioma cells is related with Tamoxifen resistance

作　　者：邹伟[1] 黄亮[1] 张骐麒 张志春[1,2] 万京华 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081 [2]中国人民解放军215医院,辽宁大连116041 [3]大连市第五人民医院神经内科,辽宁大连116029

出　　处：《辽宁师范大学学报（自然科学版）》2015年第2期225-230,共6页Journal of Liaoning Normal University:Natural Science Edition

基　　金：国家自然科学基金项目(30970353)

摘　　要：为了探讨新型雌激素受体ER-α36在他莫西芬(Tamoxifen,TAM)抑制人胶质瘤细胞增殖中的作用,以人神经胶质瘤细胞系U87-MG和U251为实验材料,采用MTT、实时定量PCR、蛋白质免疫印迹等方法,观察ER-α36的表达水平以及与TAM抗药性的关系.结果显示:(1)2种细胞中均有ER-α36的表达,但U87-MG细胞中ER-α36的表达水平明显高于U251细胞(P<0.01);(2)Tam均对2种细胞增殖产生抑制作用,且呈剂量依赖性.但U87-MG对Tam的抗药性强于U251.提示新型雌激素受体ER-α36的表达水平可能影响神经胶质瘤对TAM的抗性.In order to explore the role of novel estrogen receptor ER‐α36 in Tamoxifen（TAM） inhibi‐ting human glioma cells proliferation ,and observe the relationship between ER‐α36 and TAM resis‐tence in glioma cells ,U87‐MG and U251 cells were treated with TAM ,MTT ,Western Blot ,immu‐nofluorescence and real‐time qPCR were performed .The results were ：（1）Two kinds of human glioma cells express ER‐α36 ,but the expression of ER‐α36 in U87‐MG cells is obviously higher than that of U251 cells （P＆lt;0 .01）;（2）TAM can reduce the survival rate of both cells ,and it’s a dose‐dependent manner .The resistance of TAM in U87‐MG cells is stronger than U251 cells .We got the expression level of ER‐α36 may effect TAM resistance in glioma cells .

关 键 词：ER-α36 胶质瘤 TAMOXIFEN 

分 类 号：Q257[生物学—细胞生物学]