阿魏酸、绿原酸对血管平滑肌细胞黏附的影响  

Study on the Effects of Ferulic Acid and Cholorogenic Acid on the Vascular Smooth Muscle Cell Adhesion in vitro

作　　者：袁卓[1] 杨萃[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193

出　　处：《辽宁中医杂志》2015年第6期1288-1291,I0002,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81403332);天津市应用基础与前沿技术研究计划(13JCQNJC12600)

摘　　要：目的:研究单体阿魏酸、绿原酸对血管平滑肌细胞(vascular smooth muscle cells,VSMCs)黏附的影响,并探讨相关机制。方法:体外培养VSMCs,用单体阿魏酸、绿原酸进行干预,检测其对VSMCs与基质的黏附、VSMCs之间的黏附的影响;PT-PCR检测对基质金属蛋白酶(matrix metal loproleinase,MMP)MMP-2、MMP-9 m RNA表达的影响。结果:(1)阿魏酸(102ng/m L、103ng/m L)、绿原酸(102ng/m L、103ng/m L)对VSMCs与基质的黏附无影响;(2)阿魏酸(102ng/m L、103ng/m L)促进了VSMC之间的黏附;(3)阿魏酸(102ng/m L、103ng/m L)可下调VSMCs MMP-9 m RNA的表达。结论:阿魏酸可促进VSMC之间的黏附,这种作用是通过抑制VSMCs MMP-9 m RNA的表达实现的。Objective ： To investigate the effects of ferulic acid and eholorogenic acid on the adhesion of vascular smooth muscle cells （VSMCs） and its correlated mechanisms. Methods ： VSMCs were in vitro cultured. The VSMCs adhesion was detected after in- tervened by ferulic acid and cholorogenic acid. The effects on the mRNA expressions of matrix metalloproteinase - 2/- 9 were de- tected using RT- PCR. Results：（ 1 ）Ferulic acid （102 ng/mL, 103 ng/mL）and cholorogenic acid（ 102 ng/mL, 103 ng/mL）had no effects on VSMCs adhesion to ECM. （2） Ferulic acid （ 102 ng/mL, 103 ng/mL） promoted VSMCs and VSMCs adhesion. （3） Feru- lic acid （102 ng/mL, 103 ng/mL）could down- regulating MMP-9 mRNA expression. Conclusion ：Ferulic acid promoted VSMCs and VSMCs adhesion through down -regulating MMP- 9 mRNA expression.

关 键 词：平滑肌细胞 阿魏酸 绿原酸 细胞黏附 基质金属蛋白酶 

分 类 号：R965[医药卫生—药理学]