浊点萃取-间接光度法测定饮料中V_c  

Determination of Vitamin C in Drink by Indirec Spectrophotometric with Cloud Point Extraction

作　　者：朱圣平[1] 丁宗庆[1] 

机构地区：[1]郧阳师范高等专科学校生物化学与环境工程系,湖北十堰442000

出　　处：《食品研究与开发》2015年第9期104-106,共3页Food Research and Development

基　　金：湖北省教育厅科学技术研究项目(Q20126002);郧阳师范高等专科学校科研项目(2012A01)

摘　　要：建立了浊点萃取-间接分光光度法测定饮料中V_C的方法。优化了pH、试剂用量、平衡温度和时间等条件。该方法的线性范围4.0 mg/L^140 mg/L(r=0.999 5),检出限为2.24 mg/L,加标回收率在97.6%~102.2%之间,相对标准偏差在1.7%~2.4%之间。成功的应用于饮料样品中VC的测定。A new method for the determination of vitamin C by indirec spectrophotometric after cloud point extraction was proposed. The effects of experimental conditions such as pH value, reagent dosage, equilibration temperature and time on cloud point extraction were discussed, a good linear relationship was obtained in the range of 4.0 mg/L-140 mg/L of the vitamin C （r=0.999 5）, the detection limits of 2.24 mg/L. The recoveries fell in the range from 97.6 % to 102.2 % and the relative standard deviations were between 1.7 % and 2.4 %. This method has been applied for the determination of Vc in drink samples.

关 键 词：浊点萃取 分光光度法 V_C 分离富集 饮料 

分 类 号：O657.3[理学—分析化学] TS27[理学—化学]