西洋参叶中皂苷含量动态积累分析  

Analysis of the Dynamic Accumulation of Ginsenosides from P. quinquefolium L. Leaf

作　　者：杨正明[1] 任烨[1] 李波[1] 许云章[1] 刘圆[1] 

机构地区：[1]西南民族大学民族医药研究院,成都610041

出　　处：《中国食品学报》2015年第5期247-253,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家科技部重大新药创制科技重大专项(2011ZX09307-002-01);国家科技支撑计划项目(2012BAI27B07);国家自然科学基金项目(No.81173653)

摘　　要：目的：采用RP-HPLC-ELSD法动态测定不同生长年限的加拿大原产地西洋参叶中皂苷的含量。方法：RP-HPLC-ELSD法、主成分分析法和聚类分析法。结果：1年生西洋参叶总皂苷含量较其它生长年限的低,2~5年生西洋参叶总皂苷含量积累较多;人参皂苷Rg1、Re、Rb2、Rb1、Rc和Rg3以及拟人参皂苷F11和RT5是西洋参叶特征性的皂苷成分。按聚类分析,将1年生的叶聚为第1类,2~5年生的叶聚为第2类,且2~5年生叶的总皂苷含量趋于平稳。结论：每年9~10月割取西洋参地上部分（茎叶）,作为人参皂苷提取的植物来源。Objective： To determine the dynamic accumulation of ginsenoside in P. quinquefolium L. leaf from differ- ent grown years and different collection months in Canada. Methods： A RP-HPLC-ELSD, principal component analysis and cluster analysis were adopted. Results： The contents of one-year-old were lower than others. Principal component analysis showed that Rgl, Re, Rb2, Rb1, Rc and Rg3 were regarded as the characteristic ginsenosides of P. quirtque- folium L. leaf. Cluster analysis showed that samples of one-year-old was the first major categories, the others was the second categories. Conclusion： It was suggested that stem of Panax quinquefolium L. should be collected for extracts of ginsenoside before stem and leaf withered of Sept. to Oct. every year.

关 键 词：西洋参 叶 RP-HPLC-ELSD 人参皂苷 拟人参皂苷 主成分分析 聚类分析 

分 类 号：O657[理学—分析化学] R284.1[理学—化学]