NaOH预处理对秸秆高固体浓度酶水解效果影响  

The Effect of Na OH Pretreatment on Enzymatic Hydrolysis of Corn Straw with High Solid Concentration

作　　者：高静思 孙岩斌 文雪[3] 王玮[3] 刘研萍[3] 

机构地区：[1]深圳市清淼环保科技有限公司,广东深圳518055 [2]中国城市建设研究院有限公司,北京100120 [3]北京化工大学环境科学与工程系,北京100029

出　　处：《中国沼气》2015年第3期17-22,共6页China Biogas

基　　金：农业部公益项目(201303101);科技支撑项目(2014BAL02B03)

摘　　要：秸秆高固体条件下,酶水解有利于高浓度的还原糖产量,为了促进酶水解作用,探讨采用Na OH预处理对不同高底物浓度条件下酶水解的促进作用。结果表明：经过预处理后,酶水解还原糖总量最多增长了128%,酶水解得糖率也提高了约77%~114%,木质纤维素降解率也有较大幅度的提升,说明Na OH预处理有利于酶水解的进行,并可以促进酶水解过程。综合考虑各因素,进行高固体酶水解实验适宜的底物固体浓度应选择15%。通过对未预处理、酶水解、预处理后酶水解的玉米秸秆的微观结构分析,可以看出,预处理并酶水解后玉米秸秆的结构发生了明显的变化,纤维束表面完全被破坏;红外谱图显示预处理并酶水解后玉米秸秆与糖类有关特征峰增多,为后续厌氧消化微生物提供了更易发酵的底物;X射线衍射图显示酶水解过程对无定形区的破坏作用较大,对结晶区的水解作用较小。Na OH预处理对酶水解过程有较明显的促进作用。Enzymatic hydrolysis was beneficial to lead to high levels of sugar production under high-solid condition of corn straw. In order to promote enzymatic hydrolysis, the promoting effect of NaOH pretreatment on enzymatic hydrolysis of dif- ferent high solid substrate was studied. The results showed that, after pretreatment,the total hydrolyzed sugar increased by 128% maxlmal]y, the enzymatic hydrolytic yield increased by 77% ~ 114%, and lignocellulose degradation rate improved too. It indicated that NaOH pretreatment was conducive for enzymatic hydrolysis, and could promote the hydrolysis process. Considering of various factors, 15% was selected as the proper substrate solids concentration. The Microstructure analysis, and the showed that the structure of corn straw had significant changes after NaOH pretreatment, and the surface of the fiber bundle was completely destroyed. The FF-IR showed that, after the NaOH pretreatment and enzymatic hydrolysis, the peaks of carbohydrate increased, providing more fermentable substrate for anaerobic digestion. The X-ray diffraction indica- ted that enzymatic hydrolysis had greater destructive effect on amorphous regions than that on crystalline regions. And so, the NaOH Dretreatment had evident promoting effect on enzymatic hydrolysis.

关 键 词：预处理 酶水解 玉米秸秆 高固体浓度 还原糖 

分 类 号：TQ353[化学工程] S216.4[农业科学—农业机械化工程]