依达拉奉和远隔缺血后处理对心肌再灌注损伤的保护  

Protective effect of edaravone postconditioning and remote ischemia postconditioning on myocardial ischemia/reperfusion injury

作　　者：李知平[1] 张曙[1] 李恒昌[2] 林永锋[1] 谢海辉[1] 

机构地区：[1]广东省东莞市人民医院麻醉科,东莞523018 [2]广州市第一人民医院麻醉科

出　　处：《山西医科大学学报》2015年第6期507-510,共4页Journal of Shanxi Medical University

基　　金：广东省医学科研基金资助项目(A2012786);广州市医药卫生科技基金资助项目(201102A213081)

摘　　要：目的评价依达拉奉后处理联合远隔缺血后处理对大鼠心肌缺血再灌注损伤的影响。方法健康雄性SD大鼠40只,8周龄,体重250-300 g,采用随机数字表法,将其分为5组(n=8):假手术组(S组)、缺血再灌注组(I/R组)、依达拉奉后处理组(E组)、远隔缺血后处理组(P组)、依达拉奉联合远隔缺血后处理组(EP组)。采用结扎左冠状动脉前降支30 min,再灌注180 min制备心肌缺血再灌注模型。E组和EP组再灌注前1 min静脉注射依达拉奉3 mg/kg;P组和EP组左冠状动脉结扎20 min时实施远隔后处理(即用止血带结扎大鼠双后肢,持续10 min)。于心肌缺血再灌注末记录血浆肌酸激酶同工酶(CK-MB)、超氧化物歧化酶(SOD)、丙二醛(MDA)、心肌梗死面积测定。结果与S组相比,其他各组灌注后CK-MB和小肌梗死面积显著增加(P<0.01),I/R组灌注后SOD活力降低而MDA的水平增高,EP组灌注后SOD活力增加(P<0.05);与I/R组相比,E组、P组和EP组灌注后和小肌梗死面积减小,CK-MB、MDA的水平明显降低(P<0.01),SOD活力增加(P<0.05);EP组与E组、P组比较,CK-MB和MDA水平降低,SOD活力增加(P<0.05),心肌梗死面积明显减小(P<0.01)。结论依达拉奉后处理组和远隔缺血后处理都可减轻心肌缺血再灌注损伤,依达拉奉后处理联合远隔缺血后处理效果明显强于两者单独应用。Objective To evaluate the effects of edaravone postconditioning and remote ischemia postconditioning on myocardial ische- mia/reperfusion（I/R） injury in rats. Methods Forty male Sprague-Dawley rats, aged 8 weeks, weighing 250 -300 g, were randomly divided into 5 groups（ n = 8 in each group） ：sham operation group, I/R group, edaravone postconditioning group（ E group） ;remote is- chemia postconditioning group（ P group）, edaravone postconditioning and remote isehemia postconditioning group （EP group）. Myocar- dial I/R was induced by occlusion of anterior descending branch of left coronary artery for 30 min followed by 180 min reperfusion. Edaravone 3 mg/kg was injected intravenously at 1 rain before reperfusion in E and EP groups. The animals underwent 10 min ischemia of bilateral hind limbs after 20 min of myocardial isehemia in P and EP groups. At the end of experiment, the arterial blood was collect- ed to measure the serum levels of CK-MB, MDA, SOD and myocardial infarct size. Results Compared with sham group, CK-MB and myocardial infarct size were significantly increased in other groups after reperfusion（ P 〈 0.01 ）, SOD was decreased and MDA was increased in I/R group, and SOD was increased in EP group（P 〈0.05）. Compared with I/R group, CK-MB, MDA and myocardial infarct size were significantly decreased after reperfusion（ P 〈 0.01 ） and SOD was increased in E, P and EP groups. Compared with E and P groups, CK-MB, MDA were decreased and SOD was increased after reperfusion（ P 〈 0.05 ） , and the myocardial infarct size was significantly decreased in EP group（ P 〈 0.01 ）. Conclusion Edaravone postconditioning and remote ischemia posteonditioning can alleviate the myocardial I/R injury and offer better efficacy than either alone.

关 键 词：依达拉奉 缺血再灌注损伤 远隔缺血后处理 

分 类 号：R363[医药卫生—病理学]