青果葛根配伍液对醉酒大鼠肝脏损伤的保护作用研究  

The Protective Effects of Fructus Canarii and Puerariae Combination Treatment on Alcohol-induced Rat Liver Injury

作　　者：何鸿雁[1] 李华[2] 杨艳[1] 汪春梅[1] 陈润[1] 

机构地区：[1]泸州医学院公共卫生系,四川省泸州市646000 [2]泸州医学院药理教研室

出　　处：《医学理论与实践》2015年第12期1541-1543,共3页The Journal of Medical Theory and Practice

基　　金：四川省卫生厅项目(100217);泸州市科技局项目[泸市科函(2008)23]

摘　　要：目的:利用大鼠醉酒模型观察青果葛根配伍使用对醉酒大鼠肝匀浆中谷草转氨酶(AST)、丙氨酸转氨酶(ALT)和γ-谷氨酰转肽酶(γ-GT)活力的影响,探讨青果葛根配伍使用对醉酒大鼠肝损伤的保护作用。方法:将60只SD大鼠随机分为5组:空白对照组、模型组、青果葛根配伍使用低剂量组、青果葛根配伍使用中剂量组、青果葛根配伍使用高剂量组。采用白酒灌胃造模,连续灌胃4d后称量大鼠重量,处死,取出肝脏后将大鼠肝脏制成肝匀浆并检测AST、ALT和γ-GT活力水平。结果:模型组大鼠肝脏三项生化指标与其他各组相比差异都具有统计学意义(P<0.05);空白组及青果葛根配伍使用高、中、低剂量组肝匀浆中AST、ALT和γ-GT水平低于模型组,其差异有统计学意义(P<0.05)。结论:醉酒大鼠肝脏组织AST、ALT和γ-GT均有不同程度升高,青果葛根配伍使用对上述指标有调控作用,对肝脏具有保护作用,拥有良好的解酒功效,且其解酒功效随着剂量的增加而增加。Objective：To investigate the protective effects of fructus canarii and puerariae combination treatment on al- cohol-induced rat liver injury through testing the activities of AST, ALT and 7-GT in liver homogenates of drunk rat. Methods： Sixty Sprague-Dawley （SD） rat were divided into five groups, including blank group, model group, low-dose fructus canarii and puerariae treatment group,middle-dose fructus canarii and puerariae treatment group and high-dose fructus canarii and puerariae treatment group. After 4 days of liquor gavage, rats were sacrificed. The levels of AST, ALT and γ-GT of rat liver were measured. Results： Compared with other groups, the difference of the levels of AST, ALT and γ-GT of model group is significant （P〈0. 05）. The levels of AST, ALT and γ-GT of blank group and fructus canarii and puerariae treatment group are lower than that of model group, and the difference is significant （P〈0. 05）. Conclusion： The levels of AST, ALT and γ-GT are increased in the liver of drunk rat. Fructus canarii and puerariae combination treatment can reduce the levels of AST, ALT and γ-GT in drunk rat, and significantly inhibit liquor-induced rat liver injury. Moreover, fructus canarii and puerariae combination treatment exerts the anti-alcohol effects in an dose-de- pendent manner.

关 键 词：青果葛根配伍使用 解酒 AST ALT Γ-GT 

分 类 号：R-332[医药卫生]