突变型P53和肿瘤易感基因101在肺癌组织中的表达及意义  

Expressions of Mutant P53 and Tumor Susceptibility Gene 101 in Lung Cancer

作　　者：郭康[1] 范文艳[2] 

机构地区：[1]新乡医学院第三附属医院肿瘤科,河南新乡453003 [2]新乡医学院形态学实验室,河南新乡453003

出　　处：《河南师范大学学报（自然科学版）》2015年第3期138-142,共5页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省科技厅资助项目(122102210065);新乡市科技支持计划(09S084)

摘　　要：探讨了P53基因突变对肺癌组织中TSG101基因的影响,结果是：TSG101在正常组织中呈强阳性表达100%（30/30）,在高分化肺癌（包括肺鳞癌和肺腺癌）组织、低分化肺癌组织、淋巴结转移组织的表达分别为77.97%（92/118）、25.37%（17/67）、18.95%（18/95）;P53的表达则相反,在正常组织、高分化肺癌组织、低分化肺癌组织、淋巴结转移组织的表达分别为6.67%（2/30）、67.80%（80/118）、86.57%（58/67）、94.63%（90/95）;P53基因PCR-SSCP分析谱检测P53第5~8外显子阴性56例占30.27%,阳性129例占69.73%,总突变率为69.73%（127/180）;P53与TSG101呈现负相关.这些结果提示P53和TSG101可能共同参与了肺癌的发生、发展和转移.To investigate the effect of P53 gene mutation affecting on TSG101 gene in lung cancer tissues. Resuls： TSG101 expression was strongly positive in normal tissue, expression rate of 100% （30/30） ; In high differentiated lung cancer （including lung squamous cell carcinoma and adenocarcinoma of the lung tissue）, low differentiated lung cancer tissue, lymph node expression of metastasis tissues were 77.97% （92/118）, 25.37% （17/67）, 18.95% （18/95）. On the contrary, the ex- pression of P53 is different. Expression in normal tissue, differentiated lung cancer tissue, low differentiated lung cancer tis- sue, metastatic lymph node tissues were 6.67% （2/30）,67.80% （80/118）, 86.57% （58/67）, 94.63% （90/95）. P53 gene PCR-SSCP analysis spectya revealed that P53 exons 5- 8 were negative in 56 cases （30. 27%） and positive in 129 cases （69.73%）. The total mutation rate was 69. 73% （127/180）. P53 is negatively correlated with TSG101. These results suggest that P53 and TSG101 could be participated in the occurrence, development and metastasis of lung cancer

关 键 词：TSG101 P53 免疫组织化学 PCR-SSCP 肺癌 

分 类 号：R574.62[医药卫生—消化系统]