全自动定量胶束电动毛细管色谱检测化妆品中5种防腐剂  

Determination of Five Preservatives in Cosmetics by Fully Automated Quantitative Micellar Electrokinetic Capillary Chromatography

作　　者：徐媛[1] 凌邦瓒 姚冬 张琳 王彦[1] 阎超[1,2] 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海通微分析技术有限公司,上海201203

出　　处：《分析测试学报》2015年第6期676-680,共5页Journal of Instrumental Analysis

基　　金：国家重大科学仪器设备开发专项项目(2011YQ150072;2011YQ15007201);国家自然科学基金项目(21175092;21105064);上海市自然科学基金项目(12ZR1413600);上海市科委"科技创新行动计划"科学仪器研制(14142200200;13142200100)

摘　　要：研制了一套全自动高精度可控温定量毛细管电泳仪,通过引入10 n L的进样阀实现定量进样,配合自主研制的自动进样系统和恒温液冷系统,以提高毛细管电泳的定量重复性和准确性。采用全自动定量胶束电动毛细管色谱(q MEKC)测定市售化妆品中苯甲酸、对羟基苯甲酸甲酯(MP)、对羟基苯甲酸乙酯(EP)、对羟基苯甲酸丙酯(PP)、对羟基苯甲酸丁酯(BP)5种防腐剂的含量,优化了分析条件。以15 mmol/L硼砂-100 mmol/L SDS混合溶液(p H 9.3)为背景电解质溶液,工作电压为20 k V,5种防腐剂在该条件下得到了良好的分离和定量结果,峰面积的相对标准偏差(RSD)小于2%。该方法快速、准确,对于化妆品中防腐剂的限用量控制具有重要意义。A fully automatic quantitative capillary electrophoresis system,which consists of a labmade auto-sampler equipped with a 10 n L rotary type injector and a lab-made thermostat with cooling capacity,was developed. The accuracy and precision of the method were greatly improved compared with that of the conventional capillary electrophoresis（ CE）. This article explored the feasibility of determination of benzoic acid,methyl p-hydroxybenzoate（ MP）,ethyl p-hydroxybenzoate（ EP）,propyl p-hydroxybenzoate（ PP） and butyl p-hydroxybenzoate（ BP） in cosmetics by fully automated quantitative micellar electrokinetic capillary chromatography（ q MEKC）. The contents of MP,benzoic acid,EP,PP and BP in body cream and exfoliating gel were determined with a mobile phase of 15 mmol /L borax buffer and 100 mmol / L SDS（ p H 9. 3） mixture and a separation voltage of 20 k V. Good separation for the five preservatives was achieved under the optimal conditions,with RSDs of peak area lower than 2%. This method is rapid and accurate,and may be employed in the quality control of preservatives in cosmetics.

关 键 词：定量毛细管电泳 纳升阀 自动进样 电隔离槽 定量胶束电动毛细管色谱 防腐剂 

分 类 号：O657.75[理学—分析化学] G353.11[理学—化学]