PHBV膜与珊瑚羟基磷灰石联合修复颌骨缺损的研究  

Experimental Research on the Combination of PHBV Membrane and Coralline Hydroxyapatite on Repair of Mandibular Defects

作　　者：鲁琴艳[1] 陈黄琴[1] 黄彬[1] 冯蕊[1] 

机构地区：[1]湖北科技学院五官医学院,湖北咸宁437100

出　　处：《湖北科技学院学报（医学版）》2015年第3期204-206,F0003,共4页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北科技学院科研课题(KY10059)

摘　　要：目的通过聚羟基丁酸酯-羟基戊酸酯(PHBV)膜与珊瑚羟基磷灰石(CHA)联合修复颌骨缺损,来观察和探讨骨缺损的修复方式及成骨机制,为PHBV膜将来应用于临床提供理论依据。方法实验选择54只实验动物日本大耳白兔,随机抽取48只左侧为PHBV膜与CHA复合组(实验组),右侧为单纯CHA(对照组)。在下颌骨体部双侧造成12mm×5mm×4mm颌骨缺损,左侧缺损处CHA填充后覆盖15mm×10mm大小的PHBV膜,右侧缺损处CHA填充。另外6只兔子左右两侧各造成12mm×5mm×4mm颌骨缺损作为空白组。植入材料后在第4周、8周及12周各处死16只动物,空白组在各期处死2只,然后进行大体观察和组织学观察。结果 4周实验组缺损区中央可见新生骨组织,而对照组见大量纤维结缔组织;8周实验组缺损区中央可见多处新生骨,对照组缺损区中央可见新生骨组织,但仍可见纤维结缔组织;12周实验组缺损区新生骨组织内骨细胞小,核小,对照组骨缺损区可见部分骨组织形成。结论 PHBV膜联合CHA修复颌骨缺损,比CHA单独修复颌骨缺损成骨速度更快,新形成的骨质较好,而且PHBV膜通过膜的机械性能和维持再生空间的能力,能够阻挡成纤维细胞通透,并且能在机体内维持足够长的时间以利于新骨的形成。Objective To investigate the effect of polyhydroxybutyrate -hydroxyvalerate （ PHBV） mem-brane combined with coralline hydroxyapatite （ CHA） on maxillary defects and its mechanism .Methods Forty-eight white healthy rabbits were divided into experimental group （ left side ） and control group （ right side ） . 12mm×5mm ×4mm mandibular defect of bilateral mandibular corpus in the manufacture was made ,the PHBV membrane combined with CHA repaired defects in the experimental group , and only CHA repaired defects in the control group.Left and right sides of 12mm×5mm×4mm mandibular defects in six rabbits were used as blank group.Sixteen rabbits were sacrificed in4,8,12 weeks after operation,respectively.two in each period of control group .Results New bone could be observed in the center of defect area of experimental group after 4 weeks,but there hada large number of fibrous connective tissue in control group and new bone formation could only be seen in the bone-bed .There had many small bone cells in new bone tissue of experimental group after 12 weeks ,and some new bone cells arrange regularly as mature bone tissue .In control group ,part of bone tissue formation and the fibrous connective tissue was still visible in defect area of control group after 12 weeks.Con-clusion The combination of PHBV membrane and CHA has faster speed in bone formation of mandibular defects than that of only CHA treatment .PHBV membrane can inhibit fibrous tissue permeability and facilitate new bone formation through the mechanical property of membrane .

关 键 词：PHBV膜 CHA 颌骨缺损 

分 类 号：R783.5[医药卫生—口腔医学]