原位膀胱癌大鼠建模方法比较及超声鉴定方法  

The comparison and ultrasound diagnosis of orthotopic rat models of bladder cancer induced by BBN and MNU

作　　者：张祥[1] 许天源[1] 夏磊磊[1] 王先进[1] 秦亮[1] 朱照伟[1] 钟山[1] 邵远[1] 沈周俊[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院泌尿外科,200025

出　　处：《现代泌尿生殖肿瘤杂志》2015年第2期99-103,共5页Journal of Contemporary Urologic and Reproductive Oncology

基　　金：上海市科委实验动物研究领域科技支撑项目(12140901200)

摘　　要：目的通过对大鼠分别给予N-正丁基-N-(4-羟基-丁基)亚硝胺[N-butyl-N-(4-hydroxybutyl)nitrosamine,BBN]饮水饲养和N-甲基亚硝基脲(N-methyl-nitrosourea,MNU)膀胱灌注,来比较两种方法的造模效果以及各自的优缺点。判断B型高频超声对大鼠原位膀胱癌的诊断价值。方法BBN组大鼠给予0.05%的BBN溶液连续喂养6周,后更换为2%的枸橼酸钠溶液连续喂养22周,第28周末为诱导终点。MNU组大鼠给予MNU溶液(20mg/ml)膀胱灌注,每两周1次,每次0.1ml/只,连续灌注8周,后再常规喂养6周,第14周为诱癌终点。分别于第28周末(BBN组)和第14周末(MNU组)对两组大鼠进行超声扫描诊断,并在处死后取膀胱组织行病理学观察。比较两组的存活率和存活成瘤率。结果 BBN诱癌组20只大鼠28周后存活11只,成瘤8只,存活率为55%,存活成瘤率为72.7%。MNU组20只大鼠14周后存活18只,成瘤17只,存活率为90%,存活成瘤率为94.4%。结论利用MNU膀胱灌注行大鼠膀胱癌造模,其存活率和存活成瘤率均显著高于BBN饮水饲养造模。超声可作为大鼠膀胱癌造模活体检测的可靠方法。Objective To compare the effectiveness of construction of two orthotopic rat mod-els of bladder cancer induced by BBN and MNU,and to evaluate the value of ultrasound in the detec-ting lesions of rats with bladder cancer. Methods 20 female SD rats were given drinking water contain 0.05% BBN for 6 weeks in a row,then the replacement of 2% natrium citrate solution feed 22 weeks continuously.All the rats were evaluated by ultrasound and then killed in the 28 weeks.A total of 20 SD rats was inducted with MNU （0.1 ml per rat）by intravesical administration every other week.At 14 weeks all rats were evaluated by ultrasound and killed. Results BBN treat-ment：there were only 1 1 rats alive and 8 of them had bladder cancer 28 weeks later.All the abnor-mal changes were detected by ultrasound and the result were in accordance with the pathological ex-amination.MNU treatment：18 rats were still living in the 14 weeks.According to the images of ul-trasound ,we found that there were 1 7 rats had tumor. Conclusions The orthotopic bladder cancer model of rat can be induced by BBN and MNU.However,the mortality is lower and the tumorigenic rate is higher which were abducted by MNU than that of BBN treatment.For the orthotopic rat model with bladder cancer,the ultrasound scanning is a reliable technique and it can provide useful help for the further research.

关 键 词：膀胱肿瘤 N-正丁基-N-(4-羟基-丁基)亚硝胺 N-甲基亚硝基脲 超声检查 动物模型 

分 类 号：R737.14[医药卫生—肿瘤]