PLK1mRNA在宫颈组织中的表达与临床意义  

Expression and clinical significance of PLK1mRNA in cervical tissues

作　　者：王慧君[1] 赵静妮[1] 杨永秀[1] 刘丽娜[2] 

机构地区：[1]兰州大学第一医院妇产科,甘肃兰州730000 [2]甘肃省妇幼保健院,甘肃兰州730050

出　　处：《中国优生与遗传杂志》2015年第6期19-20,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨PLK1基因在宫颈组织中的表达情况及其与临床病理特征之间的关系。方法采用实时荧光定量RT-PCR法检测各组m RNA的表达量。计算各组表达量采用相对定量法,检测的组织中正常宫颈组(10例),宫颈上皮内瘤变组(12例),癌症组(26例)。结果随着癌的进展,各组表达量具有上升趋势,运用统计学软件和方法比较差异性表达是否有意义,结果显示,宫颈上皮内瘤变组与正常组统计学比较有意义(P<0.05),癌症组与宫颈上皮内瘤变组和正常组比较均具有统计学意义(P<0.05)。分析癌症组与某些临床特征之间的关系显示,PLK1与肿瘤细胞分化程度有关(P<0.05),与患者年龄、临床分期无关(P>0.05)。结论 PLK1与宫颈癌的发生有一定的作用,有望成为协助诊断宫颈癌的一个预测指标,并为癌的靶基因治疗提供了帮助。Objective： To investigate the relationship between expression of PLK1 gene in cervical tissues and its clinical significance. Methods： Expression of mRNA in each group was detected by real-time fluorescence quantitative RT-PCR method. Using the calculation expression of each group relative quantitative method, detection of tissue in normal cervix group （ 10 cases） , cervical intraepithelial neoplasia （12Cases） , the cancer group （26cases） . Results： With the progress of cancer, the expression level of each group has an upward trend.The use of statistical software showed cervical intraepithelial neoplasia groap and normal group compared statistically significant （P 〈0.05 ） . Cancer group and cervical intraepithelial neoplasia （CIN） group and the normal group were statistically significant （P 〈0.05） . Cancer group of relationship analysis between the display of certain clinical features showed that PLK1 and tumor cell differentiation （P〈0.05） , but the patient＇s age, clinical stage （P〉 0.05） . Conclusions： PLK1 and cervical cancer occur to a certain extent, assist in the diagnosis of cervical cancer is expected to become a predictor, and provides help for cancer gene therapy target.

关 键 词：RT-PCR法 宫颈组织 PLK1 

分 类 号：R737.33[医药卫生—肿瘤]