超声波辅助提取芹菜废弃茎叶叶绿素工艺研究  

Technology for extracting chlorophyll from discarded stems and leaves of Apium graveolens by ultrasonic- assisted method

作　　者：尹燕[1] 冯炜弘[1] 杨道兰[1] 汪建旭[1] 张艳[1] 王永林[1] 

机构地区：[1]兰州市农业科技研究推广中心,甘肃兰州730010

出　　处：《食品工业科技》2015年第13期234-237,242,共5页Science and Technology of Food Industry

基　　金：甘肃省科技支撑计划项目(1304FKCA060);兰州市科技计划项目(2012-2-139)

摘　　要：芹菜废弃茎叶中含有丰富的天然叶绿素,充分利用废弃资源,对于部分缓解紧缺的天然叶绿素资源及保护环境具有积极作用。在有机溶剂提取法的基础上采用单因素实验及Box-Behnken中心组合设计对芹菜废弃茎叶叶绿素超声波辅助提取工艺进行了优化。结果表明,用超声波辅助提取芹菜废弃茎叶叶绿素的最佳条件是:液料比20m L/g,提取时间75min,提取温度50℃。此时样品叶绿素溶出量预测值为9.796mg/g,验证值为9.629mg/g。The discarded stems and leaves of Apium graveolens are rich in natural chlorophyll,and extraction of chlorophyll from abundant Apium graveolens stems and leaves can not only alleviate the shortage of chlorophyll resources but also be beneficial for environmental protection. The chlorophyll was extracted by the method of organic-solution with the ultrasonic-assisted treatment and the Box-Behnken design combined with single factor experiments was used to optimize ultrasonic-assisted extraction of chlorophyll.The results showed that the optimal technological parameters were as follows, liquid to solid ratio of 20mL/g for ？Smin at 50℃. Under these experimental conditions, the predicted chlorophyll content was 9.796mg/g, the verification extraction yield was 9.62.gmg/g.

关 键 词：芹菜废弃茎叶 叶绿素 超声波辅助提取 响应面法 

分 类 号：TS255.36[轻工技术与工程—农产品加工及贮藏工程]