肌球蛋白Ⅱ对卵巢癌细胞增殖、迁移和侵袭的影响  

Effects of Myosin Ⅱ on the Proliferation, Migration and Invasion of Epithelial Ovarian Cancer Cells

作　　者：贺捷[1] 唐洁[1] 田文芳[1] 唐真姿[1] 陈亦乐[1] 黄斯[1] 

机构地区：[1]中南大学湘雅医学院附属肿瘤医院妇瘤一科,湖南长沙410013

出　　处：《肿瘤药学》2015年第3期179-184,共6页Anti-Tumor Pharmacy

基　　金：国家自然科学基金项目(81101996);湖南省计划发展重点科研项目(2014FJ2015)

摘　　要：目的研究肌球蛋白Ⅱ(MyosinⅡ)对人卵巢癌细胞株增殖、迁移和侵袭能力的影响。方法分别采用real-time PCR和Western blot法检测卵巢癌中MyosinⅡ的m RNA及蛋白表达;在卵巢癌细胞中转染si-MyosinⅡ和Myosin,分别下调和过表达MyosinⅡ后,采用CCK-8法检测细胞的增殖,Matrigel小室法和Transwell小室法检测细胞的迁移和侵袭能力,Western blot检测Cyclin D1和MMP9蛋白的表达水平。结果 MyosinⅡ在卵巢癌组织中的表达显著高于正常卵巢组织,且与卵巢癌的临床分期有关(P<0.05)。在卵巢癌细胞株SKOV3和Hey中分别过表达和下调MyosinⅡ后,细胞的Cydin D1和MMP-9蛋白的表达也分别出现上调和下调,且细胞的增殖、迁移和侵袭能力均相应地增加或降低,与对照组比较差异显著(P<0.05)。结论 MyosinⅡ在卵巢癌中呈显著高表达,并可促进卵巢癌细胞的增殖、迁移及侵袭。Objective To investigate the expression of Myosin Ⅱ in epithelial ovarian cancer（EOC） and it＇s effects on proliferation, migration and invasion in EOC cells. Methods We detected the m RNA and protein expression of Myosin Ⅱ in the in EOC tissue specimens by real-time polymerase chain reaction（PCR） and western blot respectively. After si RNA-mediated knockdown of Myosin Ⅱ or lentivirus-mediated overexpression of Myosin Ⅱ in EOC cell lines, CCK-8 kits were used to detect the proliferation of EOC cells. Matrigel and Transwell were used to observe cellular migration and invasion ability. Western blot were used to detect the protein expression levels of Cyclin D1 and matrix metalloproteinase-9（MMP-9）. Results Compared with normal ovarian tissues, Myosin Ⅱ in EOC was highly expressed and correlated with the corresponding clinical stage（P〈0.05）. The capacity of proliferation, migration, invasion of EOC cells with down-regulated Myosin Ⅱ were decreased with statistically significant differences, and the protein expression levels of Cyclin D1 and MMP-9 were also decreased, as compared with the control group（P〈0.001）. While overexpressed Myosin Ⅱ increased cell proliferation, migration and invasion, as well as the protein expression levels of Cyclin D1 and MMP-9, and the differences were statistically significant as compared with the control group（P〈0.001）. Conclusions Myosin Ⅱ is highly expressed in EOC and promotes the proliferation, migration and migration capability of EOC cell.

关 键 词：肌球蛋白Ⅱ 卵巢癌细胞 增殖 迁移 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]