两种硫辛酸对映体对胚胎体外发育的影响  

Effects of two kinds of lipoic acid enantiomers on early embryo development in vitro

作　　者：景原雪[1] 薛石龙[1] 王乃辉[1] 李红星[1] 孙亮[1] 宋德潇[1] 

机构地区：[1]兰州大学第一医院生殖医学专科医院,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2015年第3期27-30,共4页Journal of Lanzhou University（Medical Sciences）

基　　金：国家自然科学基金青年基金项目(81200469);甘肃省科技厅研究技术与开发专项(1205TCYA022)

摘　　要：目的探讨(R)-硫辛酸和(S)-硫辛酸对胚胎体外发育的影响。方法解冻复苏卵裂期胚胎,分别在含不同浓度(R)-硫辛酸和(S)-硫辛酸的GⅡ培养基微滴中培养,观察各组的桑胚率、致密化率、囊胚率和优质囊胚率。结果硫辛酸可提高胚胎体外发育率,(R)-硫辛酸浓度10×10-9mol/m3组桑胚率、致密化率、囊胚率和优质囊胚率分别达到了59.2%,55.1%,49.0%和54.2%,均高于其他浓度组(P<0.01)。(R)-硫辛酸浓度10×10-9mol/m3组的桑胚率、致密化率和优质胚胎率均高于(S)-硫辛酸10×10-9mol/m3组(P<0.05)。结论硫辛酸对胚胎体外发育具有促进作用,特别是对桑胚率和囊胚率具有显著的影响。(R)-硫辛酸10×10-9mol/m3组的效果最好。Objective To investigate the effects of （R）-and （S）-lipoic acid on early embryonic development. Methods The thawed and recoveried embryos of cleavage stage were cultured into GⅡmedium droplets, con-taining different density of （R）-and （S）-lipoic acid, then compared the morulaes rate, densification rate and blastocysts rate between two group in vitro. Results Lipoic acid could increase developmental rate of embryo in vitro. Morulaes, densification, blastocysts and high quality blastocysts rate of 10× 10^-9 mol/m^3 （R）-lipoic acid treated group were 59.2%, 55.1%, 49.0%and 54.2%, respectively, which were all higher than those in the other tested groups （P〈0.01）. The effect of （R）-and （S）-lipoic acid would weakened following the increased concentration. Conclusion Lipoic acid has a promoting effect on the development of embryos in vitro, espe-cially the effects on embryo morulaes and blastocysts rate, among them the （R）-lipoic acid 10 × 10^-9 mol/m^3 group is the most efficient one.

关 键 词：硫辛酸 胚胎发育 胚胎培养液 氧化应激 

分 类 号：R394.1[医药卫生—医学遗传学]