营养性复合添加剂缓解霉变玉米饲粮对仔猪空肠黏膜结构的损伤  

Nutritional Compound Additive Alleviates Jejunal Mucosal Structure Disruption of Piglets Challenged with Feed Containing Corn Contaminated with Mycotoxins

作　　者：高亿清[1] 陈代文[1] 田刚[1] 郑萍[1] 虞洁[1] 毛湘冰[1] 何军[1] 黄能金 余冰[1] 

机构地区：[1]四川农业大学动物营养研究所,动物抗病营养教育部重点实验室,雅安625014

出　　处：《动物营养学报》2015年第6期1813-1822,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：四川省科技支撑计划项目(2012NZ0001);科技部成果转化项目(2012GB2F000399);四川省成果转化项目(2013NC0053)

摘　　要：本试验旨在研究营养性复合添加剂对采食含霉变玉米饲粮断奶仔猪空肠黏膜结构与功能、氧化还原状态以及后肠微生物数量的影响。选用18头26日龄杜×长×大(DLY)断奶仔猪[(8.57±0.21)kg],随机分为3个组,每组6个重复,每个重复1头猪,分别饲喂对照组饲粮、霉变玉米组饲粮和霉变玉米+0.2%营养性复合添加剂饲粮,试验期30 d。结果发现:与对照组相比,霉变玉米极显著提高了断奶仔猪第30天血清二胺氧化酶(DAO)活性和空肠黏膜丙二醛(MDA)含量(P<0.01),显著或极显著降低了空肠黏膜总抗氧化能力(T-AOC)(P<0.01)、过氧化物歧化酶(SOD)(P<0.01)和谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05),以及盲肠和结肠中乳酸杆菌及盲肠总菌数量(P<0.01或P<0.05)。与霉变玉米组相比,霉变玉米+0.2%营养性复合添加剂显著或极显著降低了断奶仔猪第30天血清DAO活性(P<0.01)和空肠黏膜M DA含量(P<0.05),显著或极显著提高了空肠黏膜GSH-Px活性(P<0.01)及咬合蛋白(occludin)(P<0.05)、闭合小环蛋白(ZO-1)(P<0.01)和溶质转运载体家族7成员1(SLC7 A1)mRNA表达量(P<0.05)。上述结果表明,霉变玉米饲粮导致仔猪空肠通透性增加、抗氧化能力下降及后肠乳酸杆菌数量的降低,0.2%营养性复合添加剂可在一定程度缓解霉变玉米饲粮对仔猪空肠结构的损伤和脂质过氧化。This experiment was conducted to study the protective effects of nutritional compound additive on je-junal mucosal structure and function, redox status and the numbers of bacteria in caecum and colon content of weaned piglets challenged-with feed contained corn naturally contaminated with mycotoxins. Eighteen“Duroc× Landrace×Large White” piglets were randomly allocated to control group, naturally contaminated corn group and naturally contaminated corn＋0.2% nutritional compound additive group （6 replicates per group and 1 piglet per replicate） . The trial lasted for 30 d. The results showed that corn naturally contaminated with mycotoxins significantly increased the serum diamine oxidase （DAO） activity on day 30 （P〈0.01） and the content of methane dicarboxylic aldehyde （ MDA） in jejuna mucosa （ P〈0.01） , significantly decreased total antioxidation capability （ T-AOC） （ P〈0.01） , superoxide dismutase （ SOD） （ P〈0.01） and glutathione peroxidase （ GSH-Px） （ P〈0.05） activities in jejuna mucosa, as well as the number of Lactobacillus in caecum and colon chyme and the number of total bacteria in caecum chyme compared to the control group （ P〈0.05） . The 0.2% nutri-tional compound additive significantly decreased the serum DAO activity （ P〈0.01） and the content of MDA in jejuna mucosa （ P〈0.05） , significantly increaed GSH-Px activity in jejuna mucosa （ P〈0.01） , and significant-ly up-regulated the mRNA expression of jejunal occludin （ P〈0.05） , zonula-1 （ ZO-1） （ P〈0.01） and solute carrier family 7 member 1 （SLC7A1） （P〈0.05）, in comparison with the corn naturally contaminated with mycotoxins group. Collectively, these results indicate that dietary mycotoxins can increase the intestinal perme-ability, and decrease the antioxidant capacity of jejunum mucosa and the numbers of Lactobacillus in caecum and colon contents. Meanwhile, dietary supplementation with 0.2% nutritional compound additive can alleviate the d

关 键 词：断奶仔猪 营养性添加剂 霉菌毒素 肠道 

分 类 号：S816.7[农业科学—饲料科学]