城市生活污水中富油微藻的筛选  

Isolation and Screening of Oleaginous Microalgae from Municipal Wastewater

作　　者：杨婷[1,2] 任春英[3] 李娟[4] 闫艳春[2] 赵洪新[3] 吴坤[1] 王海胜[2] 

机构地区：[1]河南农业大学生命科学学院,郑州450002 [2]中国农业科学院研究生院,北京100081 [3]沈阳师范大学化学与生命科学学院,沈阳110034 [4]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《中国农业科技导报》2015年第3期144-151,共8页Journal of Agricultural Science and Technology

基　　金：公益性科研院所基本科研业务费专项(0042012003);国家自然科学基金项目(31300101)资助

摘　　要：为筛选在城市生活污水中生长快、产油高的微藻,从污水样品中分离出40株微藻,采用高通量方法(96孔板培养、尼罗红染色)从中筛选出一株优良藻株,经18S rRNA基因序列分析及形态学鉴定属于小球藻属(Chlorella)。污水培养7 d后,其油脂含量达31.21%,污水的TN、NH+4-N、TP和COD去除率分别为71.6%、98.2%、99.4%和78.8%。该藻株在污水处理与微藻生物能源生产耦合系统中具有潜在的应用前景。In order to screen out microalgae, which was fast growing with high oil productivity, 40 strains were isolated from the municipal waste-water. An excellent microalga was screened by high-throughput method （ cuhured in 96-well plates and stained by Nile red）. The isolated strain was identified as Chlorella sp. assisted by both 18S rDNA sequencing and morphological observation. After sewage cultivation for 7 d, its lipid content was 31.21%, and the removal efficiencies of total nitrogen, ammonia nitrogen, total phosphorus and COD were 71.6%, 98.2%, 99.4%, 78.8%, respectively. This microalga had the potential of application prospect in the coupling system of sewage treatment with micro-algae hioenergy production.

关 键 词：微藻分离 鉴定 油脂含量 污水处理 

分 类 号：Q949.2[生物学—植物学]