硫酸亚铁促进Clostridium acetobutylicum CICC8012发酵麦秸水解液产丁醇  

Ferrous sulfate improving buanol production of wheat straw hydrolysate by Clostridium acetobutylicum CICC8012

作　　者：李仁强[1,2,3] 靳艳玲[1,3] 郜晓峰[4] 张国华[1,3] 赵海[1,3] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]中国科学院大学,北京100049 [3]中国科学院环境与应用微生物重点实验室,成都610041 [4]河南大学药学院,开封475001

出　　处：《应用与环境生物学报》2015年第3期447-454,共8页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院"西部之光"项目(Y2C5021100)资助~~

摘　　要：以农业废弃物小麦秸秆为原料,以丙酮丁醇梭菌Clostridium acetobutylicum CICC8012为菌种发酵生产丁醇,通过单因素实验和响应面优化设计,优选硫酸亚铁对水解液进行原位脱毒,优化发酵条件并验证硫酸亚铁原位脱毒对多种原料的有效性.发酵结果显示,伴随发酵液残糖含量显著降低,溶剂产量和发酵效率显著提高.在优化的发酵条件下,初始糖浓度50 g/L,接种量10.5%,硫酸亚铁浓度0.4 g/L,发酵65 h,丁醇产量达7.33 g/L,与优化模型预测值7.35 g/L的最大丁醇产量接近,模型显著可靠.对稻草水解液、玉米秸秆水解液、标准水解液等不同原料进行处理,丁醇和总溶剂产量均得到显著提高.本研究证明直接添加硫酸亚铁对秸秆水解液丁醇发酵具有显著促进作用,很可能是一种潜在的通用增效方法,可用于提高纤维素水解液的丁醇产量.Wheat straw, a common agricultural residual, was utilized in this study as raw material to produce solvents including acetone, ethanol and butanol （ABE）. To remove the inhibition of the inhibitors in the wheat straw hydrolysate, ferrous sulfate was used for in situ detoxification of the fermentation medium, resulting in improved solvent production and fermentation efficiency, together with reduced residual sugar and intermediates concentrations. Four crucial factors including sugar concentration, inoculum ratio, ferrous sulfate concentration and fermentation time were chosen to optimize fermentation conditions with response surface methodology. The optimal conditions predicted by the model were sugar concentration of 50 g/L, inoculum ratio of 10%, ferrous sulfate concentration of 0.4 g/L and fermentation time of 65 h, to obtain butanol concentration of 7.35 g/L. The verification experiment achieved a butanol concentration of 7.33 g/L, indicating the reliability of the model. Additionally, rice straw and corn straw hydrolysate media were also detoxified with ferrous sulfate to obtain improvement similar to wheat straw hydrolysate, showing the extensive effectiveness of the detoxification method.

关 键 词：丙酮丁醇梭菌 纤维素水解液 硫酸亚铁 原位脱毒 燃料丁醇 

分 类 号：TQ923[轻工技术与工程—发酵工程]