单壁碳纳米管对Arthrobacter sp.W1生长及苯酚降解功能的影响  

Influence of single-walled carbon nanotubes on the growth and phenol biodegradation characteristics of Arthrobacter sp.W1

作　　者：李端行 王经伟[1] 沈文丽[1] 张照婧[1] 厉舒祯 李会杰[1] 刘紫嫣[1] 马桥[1] 曲媛媛[1] 周集体[1] 

机构地区：[1]大连理工大学环境学院,工业生态与环境工程教育部重点实验室,大连116024

出　　处：《应用与环境生物学报》2015年第3期483-488,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(21176040);新世纪优秀人才支持计划(NCET-13-0077);中央高校基本科研业务费专项资金(DUT14YQ107)项目资助~~

摘　　要：单壁碳纳米管(SWCNTs)对大肠杆菌等模式菌株的生长抑制作用明显,为了解SWCNTs对具有环境功能的菌株的作用,以苯酚降解菌Arthrobacter sp.W1为对象,考察不同浓度SWCNTs下菌株W1的生长曲线和苯酚降解曲线,并通过扫描电镜观察、细胞凋亡检测、DNA泄漏量分析和活性氧产量分析考察作用机制.结果表明,特定浓度范围的SWCNTs(0.5-5.0 mg/L)会加快W1的苯酚降解速率,且1.5-2.0 mg/L SWCNTs不抑制W1的生长.SWCNTs在溶液中形成团簇并吸附W1细胞,且对W1产生以物理穿刺为主的毒性作用,但在特定浓度范围的SWCNTs条件下,SWCNTs-菌株-苯酚体系增加了菌体与苯酚的接触机会,从而对W1生长和苯酚降解产生明显的促进作用.本研究结果可为进一步揭示SWCNTs的环境微生物效应提供理论依据.With the extensive production and application of single-walled carbon nanotubes （SWCNTs）, their potential effects on biological systems and human health have attracted much research attention. Most researches used Escherichia coli as model strain and observed strong antimicrobial activity of SWCNTs, rarely paying attention to the effects of SWCNTs on functional microorganisms. This research investigated the growth curves and degradation curves of a phenol- degrading bacterium Arthrobacter sp. W1 influenced by different concentrations of SWCNTs, through scanning electron microscopic observation, viability test, cellular integrity analysis and reactive oxidative stress analysis. The results showed that 0.5-5.0 mg/L SWCNTs would accelerate phenol degrading process, and 1.5-2.0 mg/L SWCNTs had no antibacterial effects on strain W1. SWCNTs aggregates would adsorb cells with the toxicity mainly caused by physical piercing. While in systems with certain concentrations of SWCNTs, the SWCNTs-cells-phenol coexisting systems would create a suitable microenvironment for W1 growth and phenol degradation. This study would provide theoretical foundation for revealing the microbial effects of SWCNTs.

关 键 词：单壁碳纳米管 苯酚 作用机制 生物降解 ARTHROBACTER sp. 

分 类 号：X172[环境科学与工程—环境科学]