构建与人类早产儿脑室周围白质软化病理相似的动物模型  

Establishing a immature rat model similar to periventricular leukomalacia in human preterm infants pathologically and neuroethologically

作　　者：邹晓萍[1] 李晓瑜[2] 

机构地区：[1]广东省佛山市第一人民医院儿科,528000 [2]中山大学附属第一医院儿科

出　　处：《中国妇幼保健》2015年第19期3295-3297,共3页Maternal and Child Health Care of China

摘　　要：目的：建立与人类早产儿脑室周围白质软化（PVL）病理相似的可靠动物模型。方法：144只2日龄（P2）sD新生大鼠，体重6～9g，雌雄不拘，随机分成正常对照组（48只），假手术组（48只）、缺氧缺血性脑损伤（HIBD）组（48只）。P2新生大鼠麻醉后经结扎左侧颈总动脉及8％浓度的氧气缺氧4h制作HIBD组。假手术组麻醉后分离左侧颈总动脉不结扎不缺氧。正常对照组未予任何处理。缺氧缺血后3天、1周、2周、4周时，各组大鼠分别麻醉后予4℃生理盐水及4％多聚甲醛心脏灌注，脑组织于4％多聚甲醛中后固定，观察脑形态学改变，脑组织石蜡切片行HE染色，病理评分。结果：HIBD组可见白质水肿、结构疏松、左侧纹状体梗死灶形成、病灶内胶质细胞浸润，丘脑及海马可见细胞凋亡。5日龄（P5）大鼠病理评分：HIBD组与正常对照组、假手术组差异有统计学意义（P〈O．01）；9日龄（P9）大鼠病理评分：HIBD组与正常对照组、假手术组差异有统计学意义（P〈0．05）；16日龄（P16）大鼠病理评分：HIBD组与正常对照组、假手术组差异有统计学意义（P〈0．01）；30日龄（P30）大鼠病理评分：HIBD组与正常对照组、假手术组差异有统计学意义（P〈0．01）。HIBD组评分均高于其他组，且呈上升趋势。结论：通过对P2新生大鼠麻醉后经结扎左侧颈总动脉及8％浓度的氧气缺氧4h，成功建立与人类早产儿PVL病理变化相似的动物模型。Objective： To establish a immature rat model of cerebral hypoxia-ischemia which was expected to be similar to periventricular leukomalaeia in human preterm infants pathologically and neuroethologically. Methods： 144 Sprague-Dawley rats （Postnatal day 2, P2） , of either sex, were randomly divided into 3 groups： normal-group, sham operation-group and HIBD-group. Every group had 48 rats. HIBD-group survived the left common carotid artery （CCA） ligation followed by 4 h exposure to 8% 02. Rats in sham operation- group only survived the left CCA isolation. Rats in normal-group was without doing anything. Brain tissues were collected at day 3, 1 week, 2 weeks, 4 weeks after HI, for Hematoxylin-Eosin staining and histological scoring. Results： In the HIBD-group, edema, rarefaction of structure were noticed in white matter, and focus of infarction and gliocyte infiltration were noticed in the left corpus striatum. P5 histological scoring： HIBD-group were significantly higher, compared that of normal-group, sham operation-group （5.75±2.44 vs 1.17±1.17, 0. 67±0. 82, P〈0.01 ） ; The difference in the P9 histological scoring between the HIBD-group and normal-group, sham operation-group had a statistical significance （7.00±3.42 vs 1.67±0. 52, 1.67±1.63, P〈0. 05） ; P16 histological scoring： HIBD-group was significantly higher, compared that of normal-group, sham operation-group （8. 38±1.92 vs 1.67±1.33, 1.33±1.21, P〈0. 01） ; The difference in the P30 histological scoring between the HIBD-group and normal-group, sham operation-group had a statistical significance （9. 88 ±0. 99 vs 0. 50 ±0. 84, 0. 67± 0. 82, P〈0. 01 ） . Conclusion： P2 rats survived the left common carotid artery （CCA） ligation followed by 4 h expo- sure to 8% 02 are similar to periventricular leukomalacia in human preterm infants pathologically and neuroethologically.

关 键 词：脑室周围白质软化 新生大鼠 动物模型 缺氧缺血 

分 类 号：R742[医药卫生—神经病学与精神病学]