贺兰山紫蘑菇萃取物抗肿瘤活性研究  

Study on Anti- tumor Activity of Cortinarius Rufo- olivaceus Extract

作　　者：方学原 鲍吉飞 苗艳霞[1] 刘金宝[1] 汪秀[1] 朱红薇[1] 

机构地区：[1]嘉兴学院生物与化学工程学院,浙江嘉兴314001

出　　处：《广州化工》2015年第12期50-52,共3页GuangZhou Chemical Industry

基　　金：国家级大学生创新创业训练计划项目(851914511)

摘　　要：以贺兰山紫蘑菇为材料,研究石油醚、乙酸乙酯、正丁醇萃取物和水提物对乳腺癌231细胞的抑制效果,通过乙醇浸提以及有机溶剂萃取等步骤得到各有机相后,采用MTT法筛选各相萃取物抗肿瘤活性。结果表明:贺兰山紫蘑菇石油醚、乙酸乙酯、正丁醇萃取物对乳腺癌231细胞均有抑制作用,石油醚萃取物效果最佳,在浓度为0.225 mg/m L时抑制率可达96.99%。而水相提取物对癌细胞抑制作用较弱。Taking Cortinarius rufo-olivaceus as materials , the inhibitory effect of petroleum ether , ethyl acetate , n-butanol extract and water extract on 231 breast cancer cells was studied.Obtained the organic phase by ethanol extraction and organic solvent extraction steps , anti-tumor activity of each phase of extracts extracted by MTT method was screened.The result showed that Cortinarius rufo-olivaceus petroleum ether , ethyl acetate and n-butanol extracts on 231 breast cancer cells had inhibitory effect , the petroleum ether extract had the best effect , inhibition rate was up to 96.99%when the concentration was 0.225 mg/mL, but the water extract had weaker inhibition of cancer cell.

关 键 词：贺兰山紫蘑菇 乙醇浸提 萃取 MTT法 乳腺癌231细胞 

分 类 号：Q28[生物学—细胞生物学]