溃疡性结肠炎大鼠结肠黏膜SOCS2、SOCS3的表达及意义  

Expressions of SOCS2 and SOCS3 in Colonic Mucosa of Rats with Ulcerative Colitis

作　　者：周婷婷[1,2] 仝巧云[1,2] 袁晋华[1,2] 

机构地区：[1]宜昌市中心人民医院消化内科 [2]三峡大学消化疾病研究所,宜昌443000

出　　处：《华中科技大学学报（医学版）》2015年第3期285-288,297,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省自然科学基金资助项目(No.2013-CFB-388)

摘　　要：目的研究溃疡性结肠炎大鼠结肠黏膜细胞因子信号转导抑制子SOCS2、SOCS3的表达,探讨其与溃疡性结肠炎的关系。方法将30只雄性SD大鼠随机均分为正常对照组、结肠炎模型组和柳氮磺胺吡啶(SASP)组,采用三硝基苯磺酸(TNBS)诱导制备溃疡性结肠炎大鼠模型。观察大鼠一般情况并评价疾病活动指数(DAI),造模7d后处死大鼠,评价其组织学损伤指数(CMDI)及肠黏膜损伤指数(TDI);采用免疫比浊法检测血清C-反应蛋白(CRP)浓度;采用RT-PCR及Western blot检测结肠组织IL-6mRNA、SOCS2、SOCS3mRNA及蛋白的表达。结果结肠炎模型组DAI、CMDI、TDI评分及CRP水平显著高于正常对照组,SASP组显著低于模型组,差异均有统计学意义(均P<0.01);结肠炎模型组IL-6 mRNA、SOCS2和SOCS3 mRNA及蛋白表达高于正常对照组(均P<0.05),SASP组IL-6 mRNA、SOCS2、SOCS3mRNA及蛋白表达低于模型组(均P<0.05)。结论溃疡性结肠炎中SOCS2、SOCS3的表达与疾病严重程度相关,随着炎症程度的加重,SOCS2、SOCS3的表达水平上调。Objective To investigate the expressions of suppressors of cytokine signaling 2（SOCS2）and 3（SOCS3）in colonic mucosa of rats with ulcerative colitis.Methods Thirty healthy male Sprague-Dawley rats were divided into normal control group（group A,n=10）,2,4,6-trinitrobenzene sulfonic acid（TNBS）-induced ulcerative colitis group（group B,n=10）and sulfasalazine（SASP）-treated group（group C,n=10）.Rats in group B and group C were induced with TNBS/ethanol enema,and general conditions and disease activity index（DAI）of all rats were observed.After 7days of administration with gastric perfusion,all rats were executed to evaluate tissues damage index（TDI）and colonic mucosa damage index（CMDI）;The concentrations of plasma C-reaction protein（CRP）were determined by immunoturbdimetry and the mRNA expressions of IL-6,SOCS2 and SOCS3in colonic tissues were assayed by means of reverse transcriptase polymerase chain reaction（RT-PCR）and the protein expressions of SOCS2 and SOCS3by Western blot.Results The DAI,TDI,CMDI and the concentrations of CRP were significantly increased in group B while compared with group A.Those were decreased in group C compared with group B（P〈0.01）.The expressions of IL-6mRNA,SOCS2 and SOCS3mRNA and proteins were significantly increased in group B as compared with group A（P〈0.05）,while those were declined in group C relative to group B（P〈0.05）.Conclusion SOCS2/3expressions were associated with the disease degree in ulcerative colitis,and they were up-regulated with the inflammation aggravation.

关 键 词：溃疡性结肠炎 三硝基苯磺酸 柳氮磺胺吡啶 细胞因子信号转导抑制子 

分 类 号：R574.1[医药卫生—消化系统]