星形细胞肿瘤中CD13和和α-SMA的表达及其与CD31阳性微血管的相互关系  

The expression of CD13 and α-SMA in astrocytic tumors and their relationship with CD31 positive microvessels

作　　者：赵占考[1] 谷彦军[1] 刘燕青[1] 何轩[1] 

机构地区：[1]武警后勤学院附属医院病理科,天津300162

出　　处：《武警后勤学院学报（医学版）》2015年第6期421-423,F0002,共4页Journal of Logistics University of PAP（Medical Sciences）

基　　金：武警后勤学院创新团队基金(WHTD201310)

摘　　要：【目的】探讨星形细胞肿瘤中CD13和α-SMA的表达、分布特点及其与CD31阳性微血管的相互关系。【方法】应用免疫组化染色方法检测60例星形细胞肿瘤和10例瘤旁脑组织中CD13、α-SMA和CD31的表达,应用免疫组化双染色方法探讨CD13或α-SMA阳性细胞与CD31阳性微血管的分布规律。【结果】在星形细胞肿瘤中CD13和α-SMA特异性的表达于血管壁细胞,CD13和CD31均阳性的微血管占CD31阳性微血管的百分比在瘤旁脑组织、弥漫型星形细胞瘤(WHOII级)、间变型星形细胞瘤(WHOIII级)及胶质母细胞瘤(WHOIV级)中分别为(93.05±6.66)、(56.98±14.03)、(30.12±10.68)和(21.75±5.22),相对应的α-SMA和CD31均阳性的微血管所占百分比分别为(95.14±10.58)、(32.84±11.13)、(18.06±5.04)和(14.27±8.08),随着WHO分级的升高均显著降低(P<0.05)。【结论】在星形细胞肿瘤中CD13和α-SMA可作为血管周细胞的特异性标记物,微血管壁结构的缺陷程度与肿瘤分级有关。[Objective]To explore the expression and distribution of CD13 and ct-SMA in astroeytic tumors and to investigate their relationship with CD31 positive microvessels. [ Methods ] Immunohistochemical staining was examined for the expression of CD 13,α- SMA and CD31 in 60 astrocytic tumors and 10 para-tumor brain tissues. Polymer double staining was adopted to detect the distribution of CD31 or α-SMA and CD31.[Results]CD13 and α-SMA were specifically expressed by vascular wall cells in astrocytic tumors. The percentages of CD13＋CD31＋ microvessels in CD31＋ microvessels were （93.05 ± 6.66）, （56.98±14.03）, （30.12 ±10.68） and （21.75 ±5.22）, in para-tumor brain tissues, diffuse astrocytomas （WHO grade II）, anaplastic astrocytoma （WHO grade III） and gliblastoma （WHO grade IV）, respectively. Correspondingly, the percentages of α-SMA＋CD31＋microvessels were （95.14 ±10.58）, （32.84 ±11.13）, （18.06 ± 5.04） and （14.27 ±8.08）, respectively. The percentages were significantly reduced with the increase of tumor grades （P〈0.05）. [Conclusiorls]CD13 and α-SMA were expressed as specific markers for pericytes in astrocytic tumors, and the degree of defects of microvessel wall was related to tumor grades.

关 键 词：星形细胞瘤 胶质母细胞瘤 微血管 CD13 CD31 Α-SMA 

分 类 号：R730.3[医药卫生—肿瘤]