骨桥蛋白与水通道蛋白-1在喉癌中的表达与临床病理特征的相关性分析  

Correlation Analysis of Clinicopathological Features with Osteopontin and Aquaporin-1 Expression in Laryngeal Carcinoma

作　　者：周波[1] 杨皖菁[1] 王勇[1] 崔勇[2] 

机构地区：[1]湖北医药学院附属东风医院耳鼻喉科,湖北十堰442000 [2]广东省人民医院,广州510080

出　　处：《现代检验医学杂志》2015年第3期60-63,共4页Journal of Modern Laboratory Medicine

基　　金：广东省医学科研基金（A2014040）.

摘　　要：目的：探讨骨桥蛋白与水通道蛋白-1在喉癌中的表达与临床病理特征的相关性。方法选择2011年8月-2014年12月湖北医药学院附属东风医院耳鼻喉科收治的80例喉癌标本，癌旁组织标本均取自癌组织0.5 cm 之外黏膜，所有标本都进行了骨桥蛋白与水通道蛋白-1表达的免疫组化分析，并与临床病理特征进行了相关性分析。结果骨桥蛋白与水通道蛋白-1的染色颗粒多位于细胞间质、淡黄色染。在癌组织中，骨桥蛋白与水通道蛋白-1阳性颗粒主要位于癌细胞的细胞浆内，弥漫性着色，靠近癌巢处着色更深。骨桥蛋白与水通道蛋白-1在喉癌组织的阳性表达率分别为70.0％和56.3％，在癌旁组织中的阳性表达率分别为5.0％和7.5％，对比差异均有统计学意义（P ＜0.05）。在80例喉癌组织标本中，骨桥蛋白与水通道蛋白-1在不同临床分期、分化程度与淋巴结转移组织中的阳性表达对比差异有统计学意义（P ＜0.05）；Spearman 相关分析显示骨桥蛋白与水通道蛋白-1在喉癌组织中的表达呈正相关（P ＜0.05）。结论骨桥蛋白与水通道蛋白-1在喉癌组织呈现高表达状况，与临床分期、分化程度与淋巴结转移有明显相关性，两者共同作用可促进肿瘤的侵袭和转移。Objective To investigate the correlation of clinicopathological features with osteopontin and aquaporin-1 expres-sion in laryngeal carcinoma.Methods From August 2011 to December 2014 in Dongfeng General Hospital,80 laryngeal cancer specimens and adjacent normal tissue samples were selected,all samples were carried out osteopontin and aquaporin Immunohistochemical analysis expression and clinicopathological characteristics and correlation analysis.Results Osteopon-tin staining particles and aquaporin-1 were located in the stromal cells,yellow dye.In cancer tissue,osteopontin and aquapor-in-1 positive granules were mainly located in the cytoplasm of cancer cells,diffuse shading,coloring deeper place near nests. The positive expression rates of osteopontin and aquaporin-1 in the laryngeal carcinoma were 70.0% and 56.3% respective-ly,the positive rates in the adjacent tissues were 5.0% and 7.5% respectively that compared to the differences were statisti-cally significant （P 〈0.05）.In 80 cases of laryngeal carcinoma.The expression of osteopontin and aquaporin-1 in different clinical stages,differentiation and lymph node metastasis tissues were statistically significant （P 〈0.05）.Spearman correla-tion analysis showed that the osteopontin and aquaporin-1 in laryngeal carcinoma were positively correlated （P 〈0.05）.Con-clusion Osteopontin and aquaporin-1 expression in laryngeal carcinoma were showed high status,and the clinical stage,de-gree of differentiation and lymph node metastasis were significantly correlated that can work together to promote tumor inva-sion and metastasis.

关 键 词：骨桥蛋白 水通道蛋白-1 喉癌 病理特征 相关性 

分 类 号：R739.65[医药卫生—肿瘤] R730.43[医药卫生—临床医学]