降糖1号水提部分提取工艺研究  

Water Fraction Extraction Process of Hypoglycemic No.1

作　　者：宋会珠 钱丽梅[1] 张建芬[2] 李越兰[1] 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]浙江树人大学生物与环境工程学院,杭州310015

出　　处：《江西中医药大学学报》2015年第3期67-69,共3页Journal of Jiangxi University of Chinese Medicine

摘　　要：目的:优选降糖1号中黄芪、地骨皮等水提部分药材的提取工艺条件。方法:建立水提液中黄芪甲苷的RP-HPLCELSD含量测定方法。采用正交试验法,以黄芪甲苷得率为指标,考察加水量、提取时间、提取次数对黄芪甲苷得率的影响,优选水提部分药材的提取工艺条件。结果:水提取部分的最优工艺条件为:12倍量水提取3次,每次1h。结论:优选得到的工艺条件,设计合理,稳定可控,有效成分得率较高。Objective：To optimize extraction process conditions of Astragalus membranaceus, cortex lycii radicis and other water extrac-tion medicinal herbs in hypoglycemic No.1.Methods：To establish a RP-HPLC-ELSD method for determination of astragaloside A in water extraction.The astragaloside A extraction rate was selected as index, using orthogonal test method, the influence of amount of water, extraction time, extraction times on the yield of astragaloside A was investigated, to optimize extraction process conditions of wa-ter extraction medicinal materials.Results：The optimum process conditions of water extraction was as follows：12 times of water and extracting for 3 times, each time is 1 hour.Conclusion： The optimized process conditions is reasonable, stable and controllable, the yield of effective components is high.

关 键 词：提取工艺 黄芪 黄芪甲苷 RP-HPLC-ELSD 

分 类 号：R284.2[医药卫生—中药学]