GLUT9基因启动子区荧光素酶表达重组体的构建  

ESTABLISHMENT OF LUCIFERASE EXPRESSION RECOMBINANT CONTAINING HUMAN GLUT9 GENE PROMOTER

作　　者：朱雪莲[1] 刘世国[1] 李长贵[1] 

机构地区：[1]青岛大学附属医院代谢性疾病科,山东青岛266003

出　　处：《青岛大学医学院学报》2015年第3期255-257,260,共4页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的构建人葡萄糖转运蛋白9(GLUT9)基因启动子区单核苷酸多态性(SNP)rs13124007位点不同单倍型(GG/CC)荧光素酶表达重组体。方法采用PCR方法扩增目的片段,产物经双酶切后克隆至表达载体pGL3-basic,并通过双酶切及测序进行验证。结果经验证,所构建质粒含有pGL3-basic全序列及GLUT9基因启动子区包含rs13124007的序列,且插入方向正确。结论 GLUT9基因启动子区荧光素酶表达重组体构建成功,为后续研究SNP rs13124007位点是否影响启动子活性奠定了基础。Objective To establish the luciferase expression recombinant containing different haplotypes DNA （GG/CC） on SNP rs13124007 of human GLUT9 gene promoter. Methods Specific DNA fragments were amplified by PCR, and after being digested by restriction endonucieases, the fragments were then inverted into the digested pGL3-basic vector. The products were verified by endonuclease and sequencing. Results The verification indicated the recombinant contained both the sequence of pGL3-basic and rs13124007 of human GLUT9 gene promoter. The segment was inserted in the right direction. Conclusion The dual luciferase expression vector is successfully constructed, which lays a foundation for subsequent research on whether SNP rs13124007 affects the activity of promoter.

关 键 词：葡萄糖转运蛋白质类 易化性 荧光素酶类 细菌 痛风 

分 类 号：R589.7[医药卫生—内分泌]