口服抗生素引起大鼠结肠炎症和多巴胺β羟化酶表达升高  

Oral antibiotics induced colon inflammation and increased expression of Dβh in rats

作　　者：陈凤[1] 王龙娟[1] 刘爽[2] 王华新[3] 范晓磊[1] 孙文长[1] 

机构地区：[1]微生物学教研室大连医科大学,辽宁大连116044 [2]劳动卫生与环境卫生学教研室大连医科大学,辽宁大连116044 [3]病理学与法医学教研室大连医科大学,辽宁大连116044

出　　处：《中国微生态学杂志》2015年第6期647-650,共4页Chinese Journal of Microecology

基　　金：辽宁省自然科学基金(2014023049)

摘　　要：目的探讨口服抗生素是否引起大鼠结肠炎症以及合成去甲肾上腺素(Norepinephrine,NE)的酶即多巴胺β羟化酶(Dopamine-β-hydroxylase,Dβh)在结肠中的变化。方法在雄性SD大鼠的饮用水中添加肠道不吸收的抗生素(新霉素、杆菌肽和游霉素),饮用1周、3周之后分别取结肠组织进行组织病理学观察,并用实时定量PCR检测大鼠结肠促炎因子白细胞介素-1β(Interleukin-1β,IL-1β)和抗炎因子白细胞介素-10(Interleukin-10,IL-10)以及Dβh mRNA的表达水平。结果与对照组相比,饮用抗生素1周大鼠结肠HE(Haematoxylin and eosin)染色显示轻微的炎症,3周时结肠炎症略有加重;饮用抗生素1周、3周大鼠结肠IL-1β的mRNA水平上升(P<0.01),IL-10的mRNA水平下降(P<0.01),而Dβh的mRNA表达在1周时,与对照组比较差异无统计学意义,3周时显著升高(P<0.05)。结论大鼠口服抗生素可引起其结肠的轻度炎症并且结肠Dβh的表达在3周时显著升高。Objective To determine whether oral antibiotics could induce colon inflammation and influence the ex- pression of dopamine-β-hydroxylase （Dβh） in rats. Methods Male SD rats were given a mixture of nonabsorbable antimicrobials （ATM, containing neomycin, bacitracin and natamycin） via drinking water for 7 or 21 days respec- tively. Pathological changes were observed and the expressions of IL-1 β, IL-10 and Dβh in the colon were assessed by real-time PCR. Results Compared with the control group, the HE staining showed mild inflammation in one- week ATM treatment group, which was slightly severer after ATM treatment for three weeks. The IL-1 β levels were increased （P 〈0. 01 ） and IL-10 levels decreased （P 〈0. 01 ） after ATM treatment for both one and three weeks. Significant difference in the expression of Dβh was not observed in the one-week group but in the three-week group （P 〈 0.05）. Conclusion Oral antibiotics can cause mild inflammation in rat colon, and the expression of Dβh in colon can significantly increase at three weeks.

关 键 词：抗生素 结肠 炎性因子 多巴胺Β羟化酶 

分 类 号：R57[医药卫生—消化系统]