亳州地区凤丹根腐病病原鉴定  

Identification of Root Rot Pathogen Paeonia ostii T.Hong et J.X.Zhang in Bozhou

作　　者：程磊[1,2] 方成武[1,2] 陈娜[1,2] 王婧[1] 叶胜明[1] 吴计划 

机构地区：[1]亳州职业技术学院药学院,安徽亳州236800 [2]安徽省中医药科学院亳州中医药研究所,安徽亳州236800 [3]北京同仁堂安徽中药材有限公司,安徽铜陵244000

出　　处：《中国农学通报》2015年第16期190-193,共4页Chinese Agricultural Science Bulletin

基　　金：国家"十二五"科技支撑计划"白芍等3种大宗药材饮片及提取物质量评价和生产过程标准研究"(2011BAI04B05);中药材生产建设项目"牡丹皮药材规范化;规模化和产业化生产基地建设"[工信部(2013)02];亳州市创新创业领军人才科研团队"中药栽培与产地加工"[亳组(2014)21]

摘　　要：为明确安徽亳州地区凤丹根腐病病原菌种类,观测了凤丹根腐病症状,通过对其病原菌分离纯化培养,依据病原菌形态特征、致病性、rDNA-ITS序列分析,鉴定病原菌种类。分离得到1种纯化菌株,其形态学特征与已报道的茄病镰刀菌(Fusarium solani)一致。在健康凤丹根部接种该分离培养的病原菌,引起的病害症状与田间症状相似。在Gen Bank进行同源性BLAST比较,该病原菌rDNA-ITS序列与4个已登录的茄病镰刀菌聚为一类,登录号为HQ176440.1、EU327190.1、HQ839783.1、JX524023.1。结果表明导致亳州栽培凤丹根腐病的病原菌为茄病镰刀菌。The aim was to identify the pathogen species of root rot on Paeonia ostii T.Hong et J.X.Zhang inBozhou City,Anhui Province.The symptoms of root rot on Paeonia ostii T.Hong et J.X.Zhang were observedand the pathogen was isolated,purified and cultured.Identification of the pathogen species was done accordingto the morphological characteristics,pathogenicity and rDNA-ITS sequence.One kind of purified strain wasobtained,which was similar to the reported Fusarium solani.The symptoms of the healthy Paeonia ostii T.Hong et J.X.Zhang root induced by inoculation with the pathogen isolate were like the symptoms in the field.Homology BLAST comparison was conducted in Gen Bank,the rDNA-ITS sequences were clustered into onegroup with four login Fusarium solani and the login ID was HQ176440.1,EU327190.1,HQ839783.1,JX524023.1,respectively.The results demonstrated that root rot on Paeonia ostii T.Hong et J.X.Zhang wascaused by Fusarium solani.

关 键 词：凤丹 根腐病 病原鉴定 茄病镰刀菌 

分 类 号：S432.1[农业科学—植物病理学]