海生红藻多管藻中藻胆蛋白的分离纯化  

Isolation and Purification of Phycobiliproteins from Marine Red Alga Polysiphonia urceolata

作　　者：郭凝[1] 王玉[1] 赵明日[1] 龚雪琴[1] 孙力[1] 

机构地区：[1]烟台大学生命科学学院,山东烟台264000

出　　处：《烟台大学学报（自然科学与工程版）》2015年第3期179-185,共7页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家自然科学基金资助项目(30571720;40976083)

摘　　要：选用Middle Fine Sephadex G-150(MFG-150)、Fine Sephadex G-150(FG-150)以及Sephacryl S-300(S-300)3种凝胶过滤对富含R-藻红蛋白(R-PE)的多管藻藻胆蛋白提取液进行R-PE、R-藻蓝蛋白(R-PC)和别藻蓝蛋白(AP)的分离,并对所得R-PE、R-PC/AP组分用DEAE Sepharose-Fast Flow离子交换层析做进一步纯化,优化建立了从多管藻藻胆蛋白提取液中同时有效分离纯化R-PE、R-PC和AP 3类藻胆蛋白组分的技术方法.R-phycerythrin （R-PE）, R-phycocyanin （R-PC） and allophycocyanin （AP） are isolated from the extrac- ted phycobiliprotein solution of Polysiphonia urceolata rich in phycoerythrin using gel filtration through Middle Fine Sephadex G-150 （MFG-150）, Fine Sephadex G-150（FG-150） and Sephaeryl S-300（S-300）. The obtained fractions of R-PE, R-PC and AP are further purified using ion-exchange chromatography through DEAE Sepharose-Fast Flow. An optimized technical procedure for simultaneous preparation of the three phycobiliprotein types from the extracted phycobiliproteins of Polysiphonia urceolata is established based on the present experiments.

关 键 词：层析 聚丙烯酰胺凝胶电泳 多管藻 藻红蛋白 藻蓝蛋白 别藻蓝蛋白 

分 类 号：Q503[生物学—生物化学]