高脂饮食诱导的肥胖对颞叶癫痫小鼠海马损伤的影响  

Effect of high fat diet induced-obesity on hippocampal damage of temporal lobe epilepsy in mice

作　　者：冯杰[1] 邓子辉[1] 张金英[1] 薛辉[1] 梁辰[1] 李建华[1] 颜光涛[2] 

机构地区：[1]解放军总医院基础所生化研究室,北京1008531 [2]解放军总医院生化科,北京1008531

出　　处：《解放军医学院学报》2015年第7期724-727,747,共5页Academic Journal of Chinese PLA Medical School

基　　金：科技基础性工作专项项目(2011FY130100);国家科技支撑计划项目(2012BAK25B01)~~

摘　　要：目的探讨肥胖对颞叶癫痫发生、发展的影响。方法高脂饲料喂养C57BL/6J小鼠建立肥胖小鼠模型,建模成功后单侧海马微量注射200 ng海人酸(kainic acid,KA)诱导颞叶癫痫,记录小鼠癫痫评分,测定血清肌酸激酶同工酶(creatine kinase isoenzyme,CK-MB)浓度,Western blot检测海马S-100β的表达。海人酸注射5周后,观察小鼠海马病理结构改变。结果海人酸注射后,肥胖小鼠的癫痫行为评分较野生型鼠显著增高;海人酸注射24 h后,与野生型鼠相比,肥胖小鼠海马血清CK-MB浓度和海马S-100β表达升高;海人酸注射后,病理切片显示肥胖鼠海马CA3区神经元丢失和星形胶质细胞活化较野生型鼠更严重,肥胖鼠星形胶质细胞形态发生明显变化。结论肥胖可能加重了小鼠对海人酸诱导颞叶癫痫的损伤。Objective To study the effect of obesity in pathogenesis of temporal lobe epilepsy. Methods A high-fat diet induced obesity model of C57BL/6J mice was established and then a normal dose of kainic acid （KA, 200 ng per mouse） were microinjected into the hippoeampus of miee to induce temporal lobe epilepsy and seizures were scored with the Racine scores. The concentration of serum CK-MB was tested and the expression of S-100 β was measured by Western blot. Then, the hippocampal pathogenesis of mice was observed 5 weeks after KA injection. Results The mean peak seizure scores of obese mice significantly increased compared with the wild mice after KA injection. After 24 h of injection of KA, the mean serum CK-MB concentration and hippocampal S-10013 expression of obese mice were higher than wild mice. The neuron loss and astrocyte activation significantly increased in hippocampal CA3 region of obese mice compared with these of the wild mice after KA injection, there were significantly morphologic change of reactive astrocyte in obese mice. Conclusion Obesity may be detrimental to KA-induced brain injury.

关 键 词：肥胖 海人酸 星形胶质细胞 颞叶癫痫 C57BL/6J小鼠 

分 类 号：R363.2[医药卫生—病理学]