酶解辅助乙醇提取牡丹种皮中的木犀草素  

Enzyme Assisted Ethanol Extraction of Luteolin in Peony Episperm

作　　者：孟庆焕[1] 祖元刚[1] 王化[2] 王洪政[1] 路祺[1] 李平 吴薇薇[1] 钟晨[1] 段喜华[1] 

机构地区：[1]森林植物生态学教育部重点实验室(东北林业大学),哈尔滨150040 [2]黑龙江省科学院自然与生态研究所 [3]哈尔滨市第三中学

出　　处：《东北林业大学学报》2015年第6期133-135,138,共4页Journal of Northeast Forestry University

基　　金：中央高校基本科研业务费专项资金项目资助(DL13BA04);国家发改委公益性行业科研专项(201404701)

摘　　要：以牡丹种皮为原料,在单因素实验基础上,利用正交试验法研究酶解辅助乙醇提取木犀草素的工艺条件。结果表明:在果胶酶0.05 g·L-1、酶解温度45℃、p H值4.5条件下进行酶解孵育180 min后,在m(牡丹种皮)∶V(乙醇)(料液比)=1 g∶10 m L、乙醇质量分数为80%、匀浆4 min条件下提取2次,木犀草素提取率最高可达3.241 mg·g-1,相对乙醇提取法木犀草素提取率提高了4.30倍。We optimized Enzyme assisted ethanol extraction of luteolin in peony episperm , and explored the effects of factors in-cluding enzymolysis temperature , enzyme content , enzymolysis pH , enzymolysis time , ethanol concentration , solid-to-liq-uid ratio, extraction temperature , and extraction time on the extraction rates of luteolin by single-factor and orthogonal test . The optimal extraction conditions were after enzyming pretreatment under pectinase content of 0.05 mg· mL-1 , enzymolysis temperature of 45℃, enzymolysis pH of 4.5, enzymolysis time of 180 min, and 80%ethanol to extract for 2 times with ex-traction time 4 min and solid-to-liquid ratio 1∶10, and the luteolin extraction was up to 3.241 mg· g-1. Compared with the traditional ethanol extraction , the luteolin yield was improved by 4.3times .

关 键 词：牡丹种皮 木犀草素 纤维素酶 果胶酶 

分 类 号：S567.15[农业科学—中草药栽培]