非综合征型唇腭裂患儿血清抗-HCV化学发光法出现假阳性的原因分析  

Causes of false-positive anti-HCV in non-syndromic cleft lip and palate with serum chemiluminescence test

作　　者：王菊英[1] 李锋[1] 周立荣[1] 唐秀英[1] 李莉[1] 

机构地区：[1]宁夏医科大学总医院医学实验中心,银川750004

出　　处：《中国免疫学杂志》2015年第6期795-797,802,共4页Chinese Journal of Immunology

基　　金：宁夏回族自治区卫生厅科学基金资助项目(201335)

摘　　要：目的：探讨非综合征型唇腭裂患儿血清丙型肝炎病毒抗体（抗-HCV）化学发光法（CLIA）出现假阳性的原因。方法：选取4 050例非综合征型唇腭裂患儿作为病例组,以同期的8 547例手术患儿为对照组,先采用CLIA检测抗-HCV;有反应性样本采用免疫印迹法（RIBA）及荧光定量PCR（FQ-PCR）确证;病例组有反应性病例又采用CLIA进行抗-HCV追踪监测至转阴;分析比较其转阴前后血清免疫球蛋白G（Ig G）、类风湿因子（RF）及自身抗体（ANA）等检测结果的差异。结果：病例组抗-HCV有反应性样本的检出率及假阳性率均高于对照组,对比差异有统计学意义（P〈0.05）;病例组检出的42例抗-HCV弱反应性样本均为假阳性,经1个月~19个月追踪监测均转为阴性,转阴前后血清Ig G、RF、ANA等检测结果,对比差异无统计学意义（P〉0.05）。结论：非综合征型唇腭裂病例抗-HCV CLIA法出现假阳性的原因可能与其血浆代谢异常产物有关,可通过追踪监测加以排除。Objective： To investigate the causes of false-positive anti-HCV in non-syndromic cleft lip and palate with serum chemiluminescence test. Methods： We performed a hospital-based,case-control study of 4 050 patients with non-syndromic cleft lip and palate and 8 547 control patients without cleft lip and palate to evaluate associations between positive anti-HCV and non-syndromic cleft lip and palate. CLIA test were used for detect anti-HCV in control group. RIBA and FQ-PCR were used for confirming the result of the positive samples of CLIA test. CLIA test were used for detect anti-HCV in case group until CLIA test was negative. Ig G,RF and ANA were used for case-control comparisons. Results： The detection rate of anti-HCV were higher in the case group than that of control group（ P〈0. 05）. The false-positive rate was 1 percent based on testing of 4 050 patients. These samples were negative after opration 1-19 months. There were no change among Ig G,RF and ANA in the false-positivepatients. Conclusion： False-positive anti-HCV in non-syndromic cleft lip was possible associated with abnormal plasma metabolic product. It can be excluded by track monitoring.

关 键 词：非综合征型唇腭裂 化学发光法 弱反应性 假阳性 追踪监测 

分 类 号：R446.61[医药卫生—诊断学]