低磷胁迫下对转AtGAPC2基因马铃薯株系的评价与筛选  

Evaluation and Selection of Transgenic AtGAPC2 Potato Test-tube Plantlets in Low Phosphorus Stress

作　　者：李锡宁 农兰霞 牟科[1] 秦春霞[1] 江廷雪 邹雪[1] 王西瑶[1] 

机构地区：[1]四川农业大学农学院,四川温江611130

出　　处：《四川农业大学学报》2015年第2期126-132,共7页Journal of Sichuan Agricultural University

基　　金：国家级大学生创新创业训练计划项目(201310626014)

摘　　要：【目的】研究转AtGAPC2基因马铃薯试管苗对低磷胁迫的响应,评价并筛选耐低磷优良株系。【方法】以26个转AtGAPC2基因马铃薯株系及其非转基因对照品种"川芋10号"的试管苗为材料,在组培体系下设置正常磷(1.25×10-3 mol/L)与低磷(1.25×10-5 mol/L)两个水平,测定相关指标。【结果】结果表明:低磷胁迫下,相比于"川芋10号",转AtGAPC2基因株系株型正常,部分表现出地上部生长势旺、根系发达、试管薯产量高等特征,转基因株系间在形态学方面亦存在差异;运用模糊隶属函数法将所有株系划分为4个级别,96.15%转AtGAPC2基因株系的隶属函数平均值高于"川芋10号",其中以第一级别中L13、L1、L3、L18、L19这5个株系表现最好;合单株块茎产量的综合值,最终筛选出L13、L1、L3这3个株系,其单株块茎产量综合值分别是56.2%、93.0%、83.1%,显著高于"川芋10号"的26.1%。【结论】转AtGAPC2基因能提高马铃薯试管苗对低磷胁迫的耐受性。[Objective] The aim of the study was to study the responses of the test-tube plantlets of transgenic AtGAPC2 potato to low phosphorus stress and to screen the excellent lines with resist- ance to low phosphorus. [Method] 26 transgenie lines and no-transgenic ＂Chuanyu 10＂ varieties were cultured in two phosphorus levels （1.25 mmol/L, 0. 0125 mmol/L）. In addition, the rele- vant indicators were determined. [Results] Compared to ＂Chuanyu 10＂, transgenic lines showed faster growth, more developed root system and higher production under low phosphorus stress. Those lines were divided into four levels by fuzzy subordination method. 96.15% transgenic lines performed better than ＂Chuanyu 10＂ did. The first level including five strains （L13, L1, L3, L18 and L19） was best. Combination with the comprehensive value of tuber yield per plant, L13, L1 and L3 three strains were eventually selected. The comprehensive value of tuber yield per plant were 56.2%, 93.0% and 83.1% respectively, which were 26.1% higher than that of ＂Chuanyu 10＂. [Conclusion] The AtGAPC2 gene can improve the tolerance of test-tube plantlets to low phosphorus stress.

关 键 词：马铃薯 转基因 AtGAPC2 耐低磷 

分 类 号：Q94[生物学—植物学]