一种含磷三足体衍生物及其铕配合物的合成及性能表征  

Synthesis and Characterization of a Phosphoruscontaining Tripod Ligand and Its Europium Comphex

作　　者：刘伟[1] 杨水兰[1] 宋盼[1] 杨天林[1] 

机构地区：[1]宁夏大学化学化工学院,能源化工重点实验室,银川750021

出　　处：《应用化学》2015年第7期777-787,共11页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金资助项目(21261018);教育部科学基金重点资助项目(208195)~~

摘　　要：设计合成了一种新型含磷的水溶性三足体衍生物[L:N-二(吡啶-二氨基乙酰基)甲基磷酸]及其Eu(Ⅲ)的配合物,用红外吸收光谱、元素分析、差热-热重和紫外光谱法等技术手段对该配合物进行了表征,用荧光光谱法研究了室温下该配合物和牛血清白蛋白(BSA)的相互作用。结果表明,配体与苦味酸铕形成1∶1型配合物Eu(pic)3L;配合物与BSA之间有很强的结合作用;配合物对BSA内源荧光的猝灭方式为静态猝灭;配合物与BSA的作用力为分子间氢键和范德华力。分别考察了Fe3+和Cu2+对配合物与BSA结合作用的影响,证明Fe3+和Cu2+能够以金属离子桥键与配合物结合使配合物-BSA的稳定性增强。根据Foster型偶极-偶极无辐射能量转移机理可知,配合物可以和BSA以偶极-偶极无辐射进行能量传递。A novel water-soluble phosphorus-containing tripod ligand [ L：N-bis（pyridine-aminoacetyl） methyl phosphate ] and its europium complexes was synthesized and characterized by elemental analysis, TG-DSC, IR spectroscopy, UV spectroscopy and molar conductance analysis. In addition, the interaction between bovine serum albumin （BSA） and complexes was investigated with fluorescence spectra at room temperature. The experimental results show that the ligand forms the 1：1 complex Eu（pic）3L with europium picrate. There exists a strong combination action between complexes and BSA. The acting forces between the complexes and BSA are hydrogen bonding and van der Waals interaction. The quenching of BSA by complexes follows the static quenching mechanism. The influence of Fe^3＋ and Cu^2 ＋ on the combination process of the complexes with BSA was investigated. It is found that Fe^3 ＋ and Cu^2＋ can strengthen the interaction of the complex with BSA through the formation a metal bridge bond with the complex. According to Foster type dipole-dipole nonradiative energy transfer mechanism, there is no radiation energy transfer between complexes and BSA by dipole-dipole.

关 键 词：含膦三脚架配体 铕配合物 牛血清白蛋白 

分 类 号：O614.3[理学—无机化学] O641.4[理学—化学]