纤连蛋白通过激活FAK促进口腔鳞癌细胞迁移的实验研究  

Experimental research of fibronectin promoting the migration of oral squamous cancer cells by mediating focal adhesion kinase

作　　者：王亚锋[1] 陈秀丽 李超[1] 何鑫[1] 黄长城[1] 毕丽霞[1] 

机构地区：[1]解放军第二炮兵总医院口腔科,北京100088 [2]解放军第二炮兵总医院人力资源部,北京100088

出　　处：《白求恩医学杂志》2015年第3期225-227,共3页Journal of Bethune Medical Science

摘　　要：目的探讨粘着斑激酶(FAK)信号通路在纤连蛋白(FN)调节口腔鳞癌细胞迁移中的作用。方法采用Transwell方法检测不同浓度FN(0,0.1,1,10和50μg/ml)对口腔鳞癌细胞迁移能力的影响,并检测FAK磷酸化抑制剂(PF-04554878)对FN促进口腔鳞癌细胞迁移能力的影响。采用Western blot法检测FN最佳浓度下的FAK活性变化。结果 FN能够促进口腔鳞癌细胞的迁移,且在浓度10μg/ml时作用最大;FN促进口腔鳞癌细胞迁移过程中FAK被激活,PF-04554878可阻断FN对口腔鳞癌细胞迁移的促进作用。结论 FN通过激活FAK促进口腔鳞癌细胞迁移。Objective To investigate the role of focal adhesion kinase （FAK） signaling pathway on the fibronectin（FN）-in-duced migration of oral squamous cancer cells in vitro .Methods The impact of FN（0,0.1,1,10 and 50 μg /ml） on oral squamous cancer cell migration ability was studied via transwell.Western blot was used to examine the activity of FAK under the optimal FN con-centration.The migration of the oral squamous cancer cells induced by FN was tested by transwell, when FAK was inhibited.Results The migration of the oral squamous cancer cells could be induced by FN, which was the most significant in 10μg /ml.FAK was acti-vated in this process and the promoting effect of FN on oral squamous cancer cell migration could be abolished by PF-04554878, an in-hibitor of FAK phosphorylation.Conclusion FN can promote the migration of oral squamous cancer cells by mediating FAK.

关 键 词：纤连蛋白 FAK PF-04554878 口腔鳞癌 

分 类 号：R739.85[医药卫生—肿瘤]