IL-13-1112C>T和CYP3A4*1G基因多态性与青霉素过敏反应的遗传相关性分析  

Genetic correlation of IL-13-1112C > T and CYP3A4*1G gene polymorphisms with penicillin allergy

作　　者：董晓慧[1] 刘欣跃[1] 李婧[1] 刘泽菁[1] 王君[1] 刘倩[1] 

机构地区：[1]兰州大学第二医院医学检验中心,兰州730000

出　　处：《临床检验杂志》2015年第5期381-384,共4页Chinese Journal of Clinical Laboratory Science

基　　金：甘肃省自然科学基金(0412A5)

摘　　要：目的探讨白介素-13(IL-13)启动子区-1112C>T及CYP3A4*1G位点基因多态性与青霉素过敏易感性的相关性。方法以甘肃地区汉族人群临床青霉素过敏反应的211例患者和220例对照为研究对象,用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测IL-13-1112C>T和CYP3A4*1G等位基因及基因型频率的遗传分布,Hardy-Weinberg检验确认标本的群体代表性,用χ2检验进行关联分析。结果 IL-13-1112C>T位点的CC、CT、TT基因型及C、T等位基因在过敏组和对照组间差异有统计学意义(χ2分别为9.280,10.257,P均<0.05);过敏组CT+TT基因型频率为50.2%,高于对照组的35.0%(χ2=10.235,P<0.05);过敏组T等位基因频率为28.9%,高于对照组的20.0%(χ2=9.280,P<0.05);且按性别分组后,男性过敏组和对照组间差异仍有统计学意义(χ2=9.215、9.525,P均<0.05)。CYP3A4*1G位点的基因型和等位基因频率在过敏组和对照组间差异均无统计学意义(χ2分别为1.169、0.360,P均>0.05)。且按性别分组后差异仍无统计学意义。结论 IL-13-1112C>T可能是青霉素药物过敏反应的易感基因,并且与男性患者的关联性更强;CYP3A4*1G基因多态性与青霉素过敏无关。Objective To evaluate the correlation of IL-13 promoter region -1112C 〉 T and CYP3A4 ＊ 1G gene polymorphisms with the susceptibility of penicillin allergy. Methods A case-control study involving in 211 patients with penicillin allergy and 220 healthy controls from Gansu province was performed. The distributions of IL-13-1112C 〉 T and CYP3A4 ＊ 1G allele and genotype frequency were determined by polymerase chain reaction-restriction fragment length polymorphism （ PCR-RFLP）, andx2 test was used for correla- tion analysis after a representative sample was confirmed by Hardy-Weinberg test. Results There were statistically significant differ- ences in the frequencies of CC, CT and TT genotypes and C and T alleles of IL-13-1112C 〉 T between the patients with penicillin aller- gy and healthy controls （X^2 = 9. 280 and 10. 257, P 〈 0.05 ）. The CT ＋ TT genotype frequency （ 50.2% ） and T allele frequency （28.9%） in the patients with penicillin allergy were significantly higher than those in healthy controls （35.0% , X^2 = 10. 235, P 〈 0.05; 20.0% , X2 = 9. 280, P 〈 0.05 ）. When stratified by gender, there was difference in males between the two groups （X^2 = 9. 215 and 9. 525, P 〈 0. 05 ） but not in females. However, there was no significant difference in the genotype and allele frequencies of CYP3A4 ＊ 1G between the two groups （X2 = 1. 169 and 0. 360, P 〉0.05）. When stratified by gender, there was also no significant difference in either males or females between the two groups. Conclusion IL-13-1112C 〉 T gene but not CYP3A4 ＊ 1G may be related to the susceptibility of penicillin allergy, and the correlation more appears in male patients.

关 键 词：青霉素 过敏反应 单核苷酸多态性 白细胞介素-13 细胞色素P450 3A4 

分 类 号：R781.67[医药卫生—口腔医学]