Hrs对结肠癌细胞凋亡及增殖作用的研究  

Effects of Hrs on Apoptosis and Cell Proliferation in Colon Cancer Cell Lines

作　　者：孙建秀[1] 李璇[1] 

机构地区：[1]中国医科大学附属第一医院血管/甲状腺外科,沈阳110001

出　　处：《中国医科大学学报》2015年第7期585-587,共3页Journal of China Medical University

基　　金：国家自然科学基金(81170295)

摘　　要：目的研究在结肠癌细胞系中Hrs与凋亡及增殖的关系。方法在结肠癌细胞系HCT-116和SW-480中下调Hrs蛋白表达,通过Incucyte、MTT法、软琼脂试验、FACS检测细胞凋亡与增殖情况。结果下调Hrs表达后,结肠癌细胞系HCT-116和SW-480凋亡明显增加,增殖及细胞活性均显著受到抑制。结论 Hrs与结肠癌细胞凋亡密切相关。Objective To study the effects of Hrs on apoptosis and cell proliferation in colon cancer cell lines HCT- 116 and SW-480. Methods The expression of Hrs in HCT- 116 and SW-480 was knocking down by shRNA. Cell apoptosis was detected by FACS and cell proliferation was de- telmined by MTF, incucyte and soft agar. Results The apoptosis was significantly increased after Hrs knocking down in both HCT-116 and SW- 480, and the cell proliferation was inhibited. Conclusion Hrs is involved in the apoptosis and proliferation of colon cancer cells.

关 键 词：HRS 结肠癌 凋亡 增殖 

分 类 号：R735[医药卫生—肿瘤]