布加综合征患者纤维化相关细胞因子的表达  

Expression of Fibrosis- related Cytokine in Budd-Chiari Syndrome

作　　者：宁欣[1] 陆召军[1] 丁晓帆[1] 桂迩[1] 高修银[1] 

机构地区：[1]徐州医学院公共卫生学院,江苏省徐州市221004

出　　处：《中国全科医学》2015年第17期2026-2030,共5页Chinese General Practice

基　　金：国家自然科学基金资助项目(81172604/H2401)

摘　　要：目的探讨纤维化相关细胞因子转化生长因子β1(TGF-β1)、血管内皮生长因子(VEGF)、缺氧诱导因子1α(HIF-1α)在布加综合征(BCS)发展至淤血性肝硬化、肝癌中的作用。方法选取2013年11月—2014年4月徐州医学院附属医院介入科收治的BCS患者47例(BCS组),肝硬化患者29例(肝硬化组),普外科患者35例(普外科组)。采集患者静脉血,并采用酶联免疫吸附法(ELISA)测定3组患者血清TGF-β1、VEGF、HIF-1α水平。结果 3组血清TGF-β1、VEGF、HIF-1α水平比较,差异均有统计学意义(P<0.05);其中肝硬化组和BCS组血清TGF-β1、VEGF、HIF-1α水平均高于普外科组,BCS组血清TGF-β1水平低于肝硬化组,VEGF、HIF-1α水平高于肝硬化组(P<0.05)。单纯BCS、BCS合并肝硬化、BCS合并肝癌患者血清TGF-β1、VEGF、HIF-1α水平比较,差异均有统计学意义(P<0.05);其中BCS合并肝硬化、BCS合并肝癌患者血清TGF-β1、VEGF、HIF-1α水平均高于单纯BCS患者,BCS合并肝癌患者血清TGF-β1、VEGF、HIF-1α水平均高于BCS合并肝硬化患者(P<0.05)。对BCS患者血清TGF-β1、VEGF、HIF-1α水平进行相关性分析,结果显示,HIF-1α与VEGF呈正相关(r=0.773,P<0.05);HIF-1α与TGF-β1呈正相关(r=0.793,P<0.05);TGF-β1与VEGF呈正相关(r=0.582,P<0.05)。结论在单纯BCS发展至淤血性肝硬化、肝癌进程中,TGF-β1、VEGF、HIF-1α表达水平增加,早期将介入治疗和抗纤维化治疗结合,对于减少淤血性肝硬化甚至肝癌的发生至关重要。Objective To explore the role of fibrosis-related cell factors,including serum transforming growth factorβ1（TGF-β1）,vascular endothelial growth factor（VEGF）and hypoxia inducible factor-1α（HIF-1α）,in the process of BCS deteriorating into congestive liver cirrhosis and liver cancer. Methods We enrolled 47 patients with BCS（ BCS group）, 29 patients with liver cirrhosis（ liver cirrhosis group） and 35 patients of the general surgery department（ the general surgery group）. The subjects were all hospitalized in the Affiliated Hospital of Xuzhou Medical College from November 2013 to April 2014. The venous blood of the subjects was sampled,and the levels of serum TGF -β1,VEGF,HIF -1α were tested by ELISA. Results The three groups were significantly（P〈0. 05）different in serum TGF-β1,VEGF and HIF-1α;the liver cirrhosis group and the BCS group were higher（P〈0. 05）than the general surgery group in TGF-β1,VEGF and HIF-1α;the BCS group was lower（P〈0. 05）in TGF-β1 and was higher（P〈0. 05）in VEGF and HIF-1α than the liver cirrhosis group. The patients with pure BCS,BCS complicated by liver cirrhosis and BCS complicated by liver cancer were significantly different（P〈0. 05） in TGF - β1,VEGF and HIF -1α;the patients with BCS complicated by liver cirrhosis and BCS complicated by liver cancer were higher（P〈0. 05）than patients with pure BCS in TGF-β1,VEGF and HIF-1α;patients with BCS combined liver cancer were higher（P〈0. 05）than patients with BCS combined liver cirrhosis in TGF-β1,VEGF and HIF-1α. The analysis of the correlation among TGF-β1,VEGF and HIF-1αin patients with BCS showed that HIF-1αwas positively correlated（r=0. 773,P〈0. 05）with VEGF,HIF-1α was positively correlated（r=0. 793,P〈0. 05）with TGF-β1,and TGF-β1 was positively correlated（r=0. 582,P〈0. 05）with VEGF. Conclusion The expression level of TGF-β1,VEGF and HIF-1α increase in the process of pure BCS deteriorating into congestive liver cirrhosis and even liver canc

关 键 词：Budd—Chiari综合征 肝硬化 肝肿瘤 转化生长因子Β1 血管内皮生长因子A 缺氧诱导因子1 Α亚基 

分 类 号：R575[医药卫生—消化系统]