类固醇雌激素分析方法研究进展  

Research advances on the analytical methods of the steroid estrogens

作　　者：黄斌[1] 孙雯雯[1] 李晓曼[1] 杨小霞[1] 任东[1] 陈蓉[1] 潘学军[1] 

机构地区：[1]昆明理工大学环境科学与工程学院,昆明650500

出　　处：《安全与环境学报》2015年第3期367-374,共8页Journal of Safety and Environment

基　　金：国家自然科学基金项目(21267012);高等学校博士学科点专项科研基金项目(20125314110003);中国博士后科学基金项目(2013M531987);云南省应用基础研究计划项目重点项目(2013FA011);云南省应用基础研究计划项目(2012FB124);中国科学院环境化学与生态毒理学国家重点实验室开放基金项目(KF2013-04)

摘　　要：类固醇雌激素因其对野生动物、人类健康及生态环境安全造成危害而受到全世界的普遍关注,并成为环境科学和生态毒理学研究的重点和热点之一。针对4种典型的类固醇雌激素(雌激素酮、17-β-雌二醇、雌三醇和17-α-乙炔基雌二醇),对其羟基和酮基衍生化技术、水和固体样品的前处理技术及仪器检测方法进行了综述,总结了类固醇雌激素分析方法的研究动态和进展。This article aims to make a research advance review over the steroid estrogens in the environmental science and ecological toxicology research field. As is known,more and more scientific and public attention has been paid to the research of the steroid estrogens due to their potential adverse effects on the wild animals,human health and ecological environment security. In this paper,we just want to make a comprehensive review over the progress and the achievements gained in the exploration of the derivatization technologies of the hydroxyl and ketone groups,the pretreating technologies of water and the solid samples,in addition to a comment over the instrument-measuring devices of the four typical steroid estrogens, such as estrone（ E1）, 17-β-estradiol（ E2）,estriol（ E3） and 17-α-ethynylestradiol（ EE2）. However,the most widely used silylation reagents are N-methyl-N-（ trimethylsilyl） trifluoroacetamide（ MSTFA） and N,O-bis（ trimethylsilyl） trifluoroacetamide（ BSTFA）,in derivating the hydroxyl and ketone groups in the steroids,which results in the formation of trimethylsilyl（ TMS） derivative. Besides,such catalysts as trimethylchlorosilane（ TMCS） and trimethylsilyl-imidazole（ TMSI）,are usually added in hoping to enhance the derivatization efficiency. What is more,since the analytical procedures available for steroids in water and solid samples usually involve time-consuming steps for pretreatment sampling,including the extraction and clean-up,scientists of the field have been trying to compare and diversify the procedures of the sampling pretreatment methods,such as liquid-liquid extraction（ LLE）,accelerated solvent extraction（ ASE）,microwave-assisted extraction（ MAE）,solid phase extraction（ SPE）,solid-phase microextraction（ SPME）etc. For example,SPE and SPME have popularly been used for cleaning water samples,whereas MAE and ASE are adopted for solid samples. In order to promote proper application of the aforementioned sample detection techniques we

关 键 词：环境学 类固醇雌激素 衍生化 样品前处理 仪器检测 

分 类 号：X132[环境科学与工程—环境科学]